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Structure paper

TitleStructural basis of human U5 snRNP late biogenesis and recycling.
Journal, issue, pagesNat Struct Mol Biol, Vol. 31, Issue 5, Page 747-751, Year 2024
Publish dateMar 11, 2024
AuthorsDaria Riabov Bassat / Supapat Visanpattanasin / Matthias K Vorländer / Laura Fin / Alexander W Phillips / Clemens Plaschka /
PubMed AbstractPre-mRNA splicing by the spliceosome requires the biogenesis and recycling of its small nuclear ribonucleoprotein (snRNP) complexes, which are consumed in each round of splicing. The human U5 snRNP ...Pre-mRNA splicing by the spliceosome requires the biogenesis and recycling of its small nuclear ribonucleoprotein (snRNP) complexes, which are consumed in each round of splicing. The human U5 snRNP is the ~1 MDa 'heart' of the spliceosome and is recycled through an unknown mechanism involving major architectural rearrangements and the dedicated chaperones CD2BP2 and TSSC4. Late steps in U5 snRNP biogenesis similarly involve these chaperones. Here we report cryo-electron microscopy structures of four human U5 snRNP-CD2BP2-TSSC4 complexes, revealing how a series of molecular events primes the U5 snRNP to generate the ~2 MDa U4/U6.U5 tri-snRNP, the largest building block of the spliceosome.
External linksNat Struct Mol Biol / PubMed:38467876 / PubMed Central
MethodsEM (single particle)
Resolution2.6 - 4.6 Å
Structure data

18229

8q7q

EMDB-18229: Structure of the recycling U5 snRNP bound to chaperones CD2BP2 and TSSC4 (State 2, Map 2)
PDB-8q7q: Structure of the recycling U5 snRNP bound to chaperones CD2BP2 and TSSC4 (State 2)
Method: EM (single particle) / Resolution: 3.2 Å

18234

8q7v

EMDB-18234: Structure of the recycling U5 snRNP bound to chaperones CD2BP2 and TSSC4 (State 1, Map 1)
PDB-8q7v: Structure of the recycling U5 snRNP bound to chaperones CD2BP2 and TSSC4 (State 1)
Method: EM (single particle) / Resolution: 3.8 Å

18235

8q7w

EMDB-18235: Structure of the recycling U5 snRNP bound to chaperone CD2BP2 (State 3, Map 3)
PDB-8q7w: Structure of the recycling U5 snRNP bound to chaperone CD2BP2 (State 3)
Method: EM (single particle) / Resolution: 3.9 Å

18237

8q7x

EMDB-18237: Structure of the recycling U5 snRNP bound to chaperone CD2BP2 (State 4, Map 4)
PDB-8q7x: Structure of the recycling U5 snRNP bound to chaperone CD2BP2 (State 4)
Method: EM (single particle) / Resolution: 4.6 Å

EMDB-18238: Structure of the recycling U5 snRNP bound to chaperone CD2BP2 and TSSC4 (Map 5)
Method: EM (single particle) / Resolution: 2.6 Å

EMDB-18239: Structure of the recycling U5 snRNP bound to chaperone CD2BP2 and TSSC4 (Map 6)
Method: EM (single particle) / Resolution: 2.7 Å

Chemicals

ChemComp-MG:
Unknown entry

ChemComp-GTP:
GUANOSINE-5'-TRIPHOSPHATE / GTP, energy-carrying molecule*YM

Source
  • homo sapiens (human)
KeywordsSPLICING / U5 snRNP / CD2BP2 / TSSC4 / spliceosome / Pre-mRNA splicing

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