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-Structure paper
Title | Self-directed assembly and clustering of the cytoplasmic domains of inwardly rectifying Kir2.1 potassium channels on association with PSD-95. |
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Journal, issue, pages | Biochim Biophys Acta, Vol. 1808, Issue 10, Page 2374-2389, Year 2011 |
Publish date | Jul 5, 2011 |
Authors | Svetlana Fomina / Tina D Howard / Olivia K Sleator / Marina Golovanova / Liam O'Ryan / Mark L Leyland / J Günter Grossmann / Richard F Collins / Stephen M Prince / |
PubMed Abstract | The interaction of the extra-membranous domain of tetrameric inwardly rectifying Kir2.1 ion channels (Kir2.1NC(4)) with the membrane associated guanylate kinase protein PSD-95 has been studied using ...The interaction of the extra-membranous domain of tetrameric inwardly rectifying Kir2.1 ion channels (Kir2.1NC(4)) with the membrane associated guanylate kinase protein PSD-95 has been studied using Transmission Electron Microscopy in negative stain. Three types of complexes were observed in electron micrographs corresponding to a 1:1 complex, a large self-enclosed tetrad complex and extended chains of linked channel domains. Using models derived from small angle X-ray scattering experiments in which high resolution structures from X-ray crystallographic and Nuclear Magnetic Resonance studies are positioned, the envelopes from single particle analysis can be resolved as a Kir2.1NC(4):PSD-95 complex and a tetrad of this unit (Kir2.1NC(4):PSD-95)(4). The tetrad complex shows the close association of the Kir2.1 cytoplasmic domains and the influence of PSD-95 mediated self-assembly on the clustering of these channels. |
External links | Biochim Biophys Acta / PubMed:21756874 |
Methods | EM (single particle) / small angle scattering |
Resolution | 17.2 - 25.8 Å |
Structure data | EMDB-1761, PDB-2xkx: EMDB-1764, PDB-2xky: EMDB-1765: EMDB-1766: |
Source |
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Keywords | STRUCTURAL PROTEIN / SCAFFOLD PROTEIN / MEMBRANE ASSOCIATED GUANYLATE KINASE / METAL TRANSPORT / ION CHANNEL / MEMBRANE PROTEIN |