Database of articles cited by EMDB/PDB/SASBDB data

Keywords
Structure methods	All X-ray diffraction NMR electron microscopy small angle scattering neutron diffraction fiber diffraction powder diffraction infrared spectroscopy EPR fluorescence transfer theoretical model Hybrid
Author
Journal
IF	>30 >20 >10 >5 all

Title	Ribosome dynamics and tRNA movement by time-resolved electron cryomicroscopy.
Journal, issue, pages	Nature, Vol. 466, Issue 7304, Page 329-333, Year 2010
Publish date	Jul 15, 2010
Authors	Niels Fischer / Andrey L Konevega / Wolfgang Wintermeyer / Marina V Rodnina / Holger Stark /
PubMed Abstract	The translocation step of protein synthesis entails large-scale rearrangements of the ribosome-transfer RNA (tRNA) complex. Here we have followed tRNA movement through the ribosome during ...The translocation step of protein synthesis entails large-scale rearrangements of the ribosome-transfer RNA (tRNA) complex. Here we have followed tRNA movement through the ribosome during translocation by time-resolved single-particle electron cryomicroscopy (cryo-EM). Unbiased computational sorting of cryo-EM images yielded 50 distinct three-dimensional reconstructions, showing the tRNAs in classical, hybrid and various novel intermediate states that provide trajectories and kinetic information about tRNA movement through the ribosome. The structures indicate how tRNA movement is coupled with global and local conformational changes of the ribosome, in particular of the head and body of the small ribosomal subunit, and show that dynamic interactions between tRNAs and ribosomal residues confine the path of the tRNAs through the ribosome. The temperature dependence of ribosome dynamics reveals a surprisingly flat energy landscape of conformational variations at physiological temperature. The ribosome functions as a Brownian machine that couples spontaneous conformational changes driven by thermal energy to directed movement.
External links	Nature / PubMed:20631791
Methods	EM (single particle)
Resolution	9.0 - 20.0 Å
Structure data	EMDB-1716: Ribosome dynamics and tRNA movement as visualized by time-resolved electron cryomicroscopy Method: EM (single particle) / Resolution: 12.0 Å EMDB-1717: Ribosome dynamics and tRNA movement as visualized by time-resolved electron cryomicroscopy Method: EM (single particle) / Resolution: 20.0 Å EMDB-1718: Ribosome dynamics and tRNA movement as visualized by time-resolved electron cryomicroscopy Method: EM (single particle) / Resolution: 17.0 Å EMDB-1719: Ribosome dynamics and tRNA movement as visualized by time-resolved electron cryomicroscopy Method: EM (single particle) / Resolution: 13.0 Å EMDB-1720: Ribosome dynamics and tRNA movement as visualized by time-resolved electron cryomicroscopy Method: EM (single particle) / Resolution: 17.0 Å EMDB-1721: Ribosome dynamics and tRNA movement as visualized by time-resolved electron cryomicroscopy Method: EM (single particle) / Resolution: 12.0 Å EMDB-1722: Ribosome dynamics and tRNA movement as visualized by time-resolved electon cryomicroscopy Method: EM (single particle) / Resolution: 17.0 Å EMDB-1723: Ribosome dynamics and tRNA movement as visualized by time-resolved electron cryomicroscopy Method: EM (single particle) / Resolution: 15.0 Å EMDB-1724: Ribosome dynamics and tRNA movement as visualized by time-resolved electron cryomicroscopy Method: EM (single particle) / Resolution: 9.0 Å EMDB-1725: Ribosome dynamics and tRNA movement as visualized by time-resolved electron cryomicroscopy Method: EM (single particle) / Resolution: 14.0 Å EMDB-1726: Ribosome dynamics and tRNA movement as visualized by time-resolved electron cryomicroscopy Method: EM (single particle) / Resolution: 11.0 Å EMDB-1727: Ribosome dynamics and tRNA movement as visualized by time-resolved electron cryomicroscopy Method: EM (single particle) / Resolution: 18.0 Å
Source	Escherichia coli (E. coli) synthetic construct (others)