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-Structure paper
Title | Structure of an archaeal RNA polymerase. |
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Journal, issue, pages | J Mol Biol, Vol. 376, Issue 2, Page 303-307, Year 2008 |
Publish date | Feb 15, 2008 |
Authors | Anselm G Kusser / Michela G Bertero / Souad Naji / Thomas Becker / Michael Thomm / Roland Beckmann / Patrick Cramer / |
PubMed Abstract | Related multisubunit RNA polymerases (RNAPs) carry out gene transcription in all kingdoms of life. Since structural information is limited to bacterial and eukaryotic RNAPs, we determined the cryo- ...Related multisubunit RNA polymerases (RNAPs) carry out gene transcription in all kingdoms of life. Since structural information is limited to bacterial and eukaryotic RNAPs, we determined the cryo-electron microscopic structure of the RNAP from the thermophilic archaeon Pyrococcus furiosus at 13 A resolution. Comparison with eukaryotic RNAP II reveals a conserved architecture, no homologues for subunits Rpb8 and Rpb9, and significant deviation in the polymerase foot, jaws, pore, and protrusion. The structural organization of the archaeal RNA polymerase serves as a reference for future structure-function analysis of the transcription mechanism and allows for evolutionary comparisons. |
External links | J Mol Biol / PubMed:18164030 |
Methods | EM (single particle) |
Resolution | 13.0 Å |
Structure data | EMDB-1711: |
Source |
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