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TitleThe bacterial replication origin BUS promotes nucleobase capture.
Journal, issue, pagesNat Commun, Vol. 14, Issue 1, Page 8339, Year 2023
Publish dateDec 14, 2023
AuthorsSimone Pelliciari / Salomé Bodet-Lefèvre / Stepan Fenyk / Daniel Stevens / Charles Winterhalter / Frederic D Schramm / Sara Pintar / Daniel R Burnham / George Merces / Tomas T Richardson / Yumiko Tashiro / Julia Hubbard / Hasan Yardimci / Aravindan Ilangovan / Heath Murray /
PubMed AbstractGenome duplication is essential for the proliferation of cellular life and this process is generally initiated by dedicated replication proteins at chromosome origins. In bacteria, DNA replication is ...Genome duplication is essential for the proliferation of cellular life and this process is generally initiated by dedicated replication proteins at chromosome origins. In bacteria, DNA replication is initiated by the ubiquitous DnaA protein, which assembles into an oligomeric complex at the chromosome origin (oriC) that engages both double-stranded DNA (dsDNA) and single-stranded DNA (ssDNA) to promote DNA duplex opening. However, the mechanism of DnaA specifically opening a replication origin was unknown. Here we show that Bacillus subtilis DnaA assembles into a continuous oligomer at the site of DNA melting, extending from a dsDNA anchor to engage a single DNA strand. Within this complex, two nucleobases of each ssDNA binding motif (DnaA-trio) are captured within a dinucleotide binding pocket created by adjacent DnaA proteins. These results provide a molecular basis for DnaA specifically engaging the conserved sequence elements within the bacterial chromosome origin basal unwinding system (BUS).
External linksNat Commun / PubMed:38097584 / PubMed Central
MethodsEM (single particle) / X-ray diffraction
Resolution2.38 - 3.2 Å
Structure data

16229

8btg

EMDB-16229, PDB-8btg:
Cryo-EM structure of the bacterial replication origin opening basal unwinding system
Method: EM (single particle) / Resolution: 3.2 Å

PDB-8bv3:
Bacillus subtilis DnaA domain III structure
Method: X-RAY DIFFRACTION / Resolution: 2.38 Å

Chemicals

ChemComp-MG:
Unknown entry

ChemComp-ATP:
ADENOSINE-5'-TRIPHOSPHATE / ATP, energy-carrying molecule*YM

ChemComp-ANP:
PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER / AMP-PNP, energy-carrying molecule analogue*YM

ChemComp-K:
Unknown entry

ChemComp-HOH:
WATER

Source
  • bacillus subtilis (bacteria)
  • dna molecule (others)
KeywordsREPLICATION / DnaA / cryo-EM / BUS complex / replication initiation / CELL CYCLE / DNA replication / DNA replication initiation / AAA+ superfamily / Initiator Clade

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