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-Structure paper
Title | Structure and uncoating of immature adenovirus. |
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Journal, issue, pages | J Mol Biol, Vol. 392, Issue 2, Page 547-557, Year 2009 |
Publish date | Sep 18, 2009 |
Authors | Ana J Pérez-Berná / Roberto Marabini / Sjors H W Scheres / Rosa Menéndez-Conejero / Igor P Dmitriev / David T Curiel / Walter F Mangel / S Jane Flint / Carmen San Martín / |
PubMed Abstract | Maturation via proteolytic processing is a common trait in the viral world and is often accompanied by large conformational changes and rearrangements in the capsid. The adenovirus protease has been ...Maturation via proteolytic processing is a common trait in the viral world and is often accompanied by large conformational changes and rearrangements in the capsid. The adenovirus protease has been shown to play a dual role in the viral infectious cycle: (a) in maturation, as viral assembly starts with precursors to several of the structural proteins but ends with proteolytically processed versions in the mature virion, and (b) in entry, because protease-impaired viruses have difficulties in endosome escape and uncoating. Indeed, viruses that have not undergone proteolytic processing are not infectious. We studied the three-dimensional structure of immature adenovirus particles as represented by the adenovirus type 2 thermosensitive mutant ts1 grown under non-permissive conditions and compared it with the mature capsid. Our three-dimensional electron microscopy maps at subnanometer resolution indicate that adenovirus maturation does not involve large-scale conformational changes in the capsid. Difference maps reveal the locations of unprocessed peptides pIIIa and pVI and help define their role in capsid assembly and maturation. An intriguing difference appears in the core, indicating a more compact organization and increased stability of the immature cores. We have further investigated these properties by in vitro disassembly assays. Fluorescence and electron microscopy experiments reveal differences in the stability and uncoating of immature viruses, both at the capsid and core levels, as well as disassembly intermediates not previously imaged. |
External links | J Mol Biol / PubMed:19563809 / PubMed Central |
Methods | EM (single particle) |
Resolution | 8.7 - 8.9 Å |
Structure data | EMDB-1579: EMDB-1586: |