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TitleStructure of a volume-regulated heteromeric LRRC8A/C channel.
Journal, issue, pagesNat Struct Mol Biol, Vol. 30, Issue 1, Page 52-61, Year 2023
Publish dateDec 15, 2022
AuthorsSonja Rutz / Dawid Deneka / Antje Dittmann / Marta Sawicka / Raimund Dutzler /
PubMed AbstractVolume-regulated anion channels (VRACs) participate in the cellular response to osmotic swelling. These membrane proteins consist of heteromeric assemblies of LRRC8 subunits, whose compositions ...Volume-regulated anion channels (VRACs) participate in the cellular response to osmotic swelling. These membrane proteins consist of heteromeric assemblies of LRRC8 subunits, whose compositions determine permeation properties. Although structures of the obligatory LRRC8A, also referred to as SWELL1, have previously defined the architecture of VRACs, the organization of heteromeric channels has remained elusive. Here we have addressed this question by the structural characterization of murine LRRC8A/C channels. Like LRRC8A, these proteins assemble as hexamers. Despite 12 possible arrangements, we find a predominant organization with an A:C ratio of two. In this assembly, four LRRC8A subunits cluster in their preferred conformation observed in homomers, as pairs of closely interacting proteins that stabilize a closed state of the channel. In contrast, the two interacting LRRC8C subunits show a larger flexibility, underlining their role in the destabilization of the tightly packed A subunits, thereby enhancing the activation properties of the protein.
External linksNat Struct Mol Biol / PubMed:36522427 / PubMed Central
MethodsEM (single particle) / X-ray diffraction
Resolution3.1 - 18.2 Å
Structure data

15835

8b40

EMDB-15835: Cryo-EM structure of homomeric LRRC8C Volume-Regulated Anion Channel
PDB-8b40: Structure of homomeric LRRC8C Volume-Regulated Anion Channel
Method: EM (single particle) / Resolution: 4.6 Å

15836

8b41

EMDB-15836: Cryo-EM structure of heteromeric LRRC8A/C (1:1 co-transfected) Volume-Regulated Anion Channel in complex with synthetic nanobody Sb1
PDB-8b41: Structure of heteromeric LRRC8A/C (1:1 co-transfected) Volume-Regulated Anion Channel in complex with synthetic nanobody Sb1
Method: EM (single particle) / Resolution: 3.8 Å

15837

8b42

EMDB-15837: Cryo-EM structure of heteromeric LRRC8A/C Volume-Regulated Anion Channel
PDB-8b42: Structure of heteromeric LRRC8A/C Volume-Regulated Anion Channel.
Method: EM (single particle) / Resolution: 6.6 Å

EMDB-15838: Cryo-EM structure of heteromeric LRRC8A/C (1:3 co-transfected) Volume-Regulated Anion Channel in complex with synthetic nanobody Sb1
Method: EM (single particle) / Resolution: 9.5 Å

EMDB-15839: Cryo-EM structure of heteromeric LRRC8A/C (endogenous) Volume-Regulated Anion Channel in complex with synthetic nanobody Sb1
Method: EM (single particle) / Resolution: 18.2 Å

EMDB-15840: Cryo-EM structure of homomeric LRRC8A_SAM Volume-Regulated Anion Channel
Method: EM (single particle) / Resolution: 6.9 Å

EMDB-15841: Cryo-EM structure of heteromeric LRRC8A_SAM/C Volume-Regulated Anion Channel
Method: EM (single particle) / Resolution: 7.8 Å

PDB-8ben:
LRR domain Structure of the LRRC8C protein
Method: X-RAY DIFFRACTION / Resolution: 3.1 Å

Source
  • mus musculus (house mouse)
  • synthetic construct (others)
  • Homo sapiens (human)
KeywordsMEMBRANE PROTEIN / Ion channel / Volume-regulated anion channel

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