Database of articles cited by EMDB/PDB/SASBDB data

Keywords
Structure methods	All X-ray diffraction NMR electron microscopy small angle scattering neutron diffraction fiber diffraction powder diffraction infrared spectroscopy EPR fluorescence transfer theoretical model Hybrid
Author
Journal
IF	>30 >20 >10 >5 all

Title	Filament structure and subcellular organization of the bacterial intermediate filament-like protein crescentin.
Journal, issue, pages	Proc Natl Acad Sci U S A, Vol. 121, Issue 7, Page e2309984121, Year 2024
Publish date	Feb 13, 2024
Authors	Yue Liu / Fusinita van den Ent / Jan Löwe /
PubMed Abstract	The protein crescentin is required for the crescent shape of the freshwater bacterium (). Crescentin forms a filamentous structure on the inner, concave side of the curved cells. It shares features ...The protein crescentin is required for the crescent shape of the freshwater bacterium (). Crescentin forms a filamentous structure on the inner, concave side of the curved cells. It shares features with eukaryotic intermediate filament (IF) proteins, including the formation of static filaments based on long and parallel coiled coils, the protein's length, structural roles in cell and organelle shape determination and the presence of a coiled coil discontinuity called the "stutter." Here, we have used electron cryomicroscopy (cryo-EM) to determine the structure of the full-length protein and its filament, exploiting a crescentin-specific nanobody. The filament is formed by two strands, related by twofold symmetry, that each consist of two dimers, resulting in an octameric assembly. Crescentin subunits form longitudinal contacts head-to-head and tail-to-tail, making the entire filament non-polar. Using in vivo site-directed cysteine cross-linking, we demonstrated that contacts observed in the in vitro filament structure exist in cells. Electron cryotomography (cryo-ET) of cells expressing crescentin showed filaments on the concave side of the curved cells, close to the inner membrane, where they form a band. When comparing with current models of IF proteins and their filaments, which are also built from parallel coiled coil dimers and lack overall polarity, it emerges that IF proteins form head-to-tail longitudinal contacts in contrast to crescentin and hence several inter-dimer contacts in IFs have no equivalents in crescentin filaments. Our work supports the idea that intermediate filament-like proteins achieve their shared polymerization and mechanical properties through a variety of filament architectures.
External links	Proc Natl Acad Sci U S A / PubMed:38324567 / PubMed Central
Methods	EM (single particle)
Resolution	3.3 - 6.9 Å
Structure data	15395 8afe EMDB-15395, PDB-8afe: Cryo-EM structure of crescentin filaments (stutter mutant, C1 symmetry and small box) Method: EM (single particle) / Resolution: 3.3 Å 15398 8afh EMDB-15398, PDB-8afh: Cryo-EM structure of crescentin filaments (stutter mutant, C2, symmetry and small box) Method: EM (single particle) / Resolution: 3.9 Å EMDB-15399: Cryo-EM map of crescentin filament in complex with a megabody (stutter mutant, C2 symmetry, large box) Method: EM (single particle) / Resolution: 4.1 Å EMDB-15400: Cryo-EM map of crescentin filaments in complex with a megabody (wildtype, C2, large box) Method: EM (single particle) / Resolution: 6.9 Å 15401 8afl EMDB-15401, PDB-8afl: Cryo-EM structure of crescentin filaments (wildtype, C1 symmetry and small box) Method: EM (single particle) / Resolution: 4.4 Å 15402 8afm EMDB-15402, PDB-8afm: Cryo-EM structure of crescentin filaments (wildtype, C2 symmetry and small box) Method: EM (single particle) / Resolution: 4.8 Å 15446 8ahl EMDB-15446, PDB-8ahl: Cryo-EM structure of crescentin filaments (stutter mutant, C1 symmetry and large box) Method: EM (single particle) / Resolution: 4.1 Å 15465 8aia EMDB-15465, PDB-8aia: Cryo-EM structure of crescentin filaments (wildtype, C1 symmetry and large box) Method: EM (single particle) / Resolution: 5.1 Å 15473 8aix EMDB-15473, PDB-8aix: Cryo-EM structure of crescentin filaments (wildtype, C2 symmetry and large box) Method: EM (single particle) / Resolution: 5.8 Å 15476 8ajb EMDB-15476, PDB-8ajb: Cryo-EM structure of crescentin filaments (stutter mutant, C2 symmetry and large box) Method: EM (single particle) / Resolution: 4.3 Å
Source	caulobacter vibrioides (bacteria) camelidae (mammal)
Keywords	STRUCTURAL PROTEIN / cytoskeleton / cell shape / intermediate filaments / coiled coil / assembly