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-Structure paper
Title | The structure of a polyketide synthase bimodule core. |
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Journal, issue, pages | Sci Adv, Vol. 8, Issue 38, Page eabo6918, Year 2022 |
Publish date | Sep 23, 2022 |
Authors | Yves U Tittes / Dominik A Herbst / Solène F X Martin / Hugo Munoz-Hernandez / Roman P Jakob / Timm Maier / |
PubMed Abstract | Polyketide synthases (PKSs) are predominantly microbial biosynthetic enzymes. They assemble highly potent bioactive natural products from simple carboxylic acid precursors. The most versatile ...Polyketide synthases (PKSs) are predominantly microbial biosynthetic enzymes. They assemble highly potent bioactive natural products from simple carboxylic acid precursors. The most versatile families of PKSs are organized as assembly lines of functional modules. Each module performs one round of precursor extension and optional modification, followed by directed transfer of the intermediate to the next module. While enzymatic domains and even modules of PKSs are well understood, the higher-order modular architecture of PKS assembly lines remains elusive. Here, we visualize a PKS bimodule core using cryo-electron microscopy and resolve a two-dimensional meshwork of the bimodule core formed by homotypic interactions between modules. The sheet-like organization provides the framework for efficient substrate transfer and for sequestration of trans-acting enzymes required for polyketide production. |
External links | Sci Adv / PubMed:36129979 / PubMed Central |
Methods | EM (single particle) / X-ray diffraction |
Resolution | 1.62 - 6.8 Å |
Structure data | EMDB-14793, PDB-7zma: EMDB-14795, PDB-7zmd: EMDB-14945: K3DAK4 bimodule core of BGC11 from Brevibacillus brevis PDB-7zm9: PDB-7zmc: PDB-7zmf: |
Chemicals | ChemComp-PEG: ChemComp-GOL: ChemComp-HOH: ChemComp-MG: |
Source |
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Keywords | BIOSYNTHETIC PROTEIN / Ketosynthase / polyketide synthase / Claisen condensation / thiolase fold / Dehydratase / Double hot dog fold |