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TitleMolecular Organisation of Tick-Borne Encephalitis Virus.
Journal, issue, pagesViruses, Vol. 14, Issue 4, Year 2022
Publish dateApr 11, 2022
AuthorsLauri I A Pulkkinen / Sarah V Barrass / Aušra Domanska / Anna K Överby / Maria Anastasina / Sarah J Butcher /
PubMed AbstractTick-borne encephalitis virus (TBEV) is a pathogenic, enveloped, positive-stranded RNA virus in the family . Structural studies of flavivirus virions have primarily focused on mosquito-borne species, ...Tick-borne encephalitis virus (TBEV) is a pathogenic, enveloped, positive-stranded RNA virus in the family . Structural studies of flavivirus virions have primarily focused on mosquito-borne species, with only one cryo-electron microscopy (cryo-EM) structure of a tick-borne species published. Here, we present a 3.3 Å cryo-EM structure of the TBEV virion of the Kuutsalo-14 isolate, confirming the overall organisation of the virus. We observe conformational switching of the peripheral and transmembrane helices of M protein, which can explain the quasi-equivalent packing of the viral proteins and highlights their importance in stabilising membrane protein arrangement in the virion. The residues responsible for M protein interactions are highly conserved in TBEV but not in the structurally studied Hypr strain, nor in mosquito-borne flaviviruses. These interactions may compensate for the lower number of hydrogen bonds between E proteins in TBEV compared to the mosquito-borne flaviviruses. The structure reveals two lipids bound in the E protein which are important for virus assembly. The lipid pockets are comparable to those recently described in mosquito-borne Zika, Spondweni, Dengue, and Usutu viruses. Our results thus advance the understanding of tick-borne flavivirus architecture and virion-stabilising interactions.
External linksViruses / PubMed:35458522 / PubMed Central
MethodsEM (single particle)
Resolution3.3 - 3.5 Å
Structure data

14512

7z51

EMDB-14512, PDB-7z51:
Tick-borne encephalitis virus Kuutsalo-14
Method: EM (single particle) / Resolution: 3.3 Å

EMDB-14516: Tick-Borne Encephalitis virus Kuutsalo-14
Method: EM (single particle) / Resolution: 3.5 Å

Chemicals

ChemComp-CPL:
1-PALMITOYL-2-LINOLEOYL-SN-GLYCERO-3-PHOSPHOCHOLINE / phospholipid*YM

Source
  • tick-borne encephalitis virus (western subtype)
KeywordsVIRUS / virion / membrane protein / glycoprotein

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