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TitleStructure of the mycobacterial ESX-5 type VII secretion system pore complex.
Journal, issue, pagesSci Adv, Vol. 7, Issue 26, Year 2021
Publish dateJun 25, 2021
AuthorsKatherine S H Beckham / Christina Ritter / Grzegorz Chojnowski / Daniel S Ziemianowicz / Edukondalu Mullapudi / Mandy Rettel / Mikhail M Savitski / Simon A Mortensen / Jan Kosinski / Matthias Wilmanns /
PubMed AbstractThe ESX-5 type VII secretion system is a membrane-spanning protein complex key to the virulence of mycobacterial pathogens. However, the overall architecture of the fully assembled translocation ...The ESX-5 type VII secretion system is a membrane-spanning protein complex key to the virulence of mycobacterial pathogens. However, the overall architecture of the fully assembled translocation machinery and the composition of the central secretion pore have remained unknown. Here, we present the high-resolution structure of the 2.1-megadalton ESX-5 core complex. Our structure captured a dynamic, secretion-competent conformation of the pore within a well-defined transmembrane section, sandwiched between two flexible protein layers at the cytosolic entrance and the periplasmic exit. We propose that this flexibility endows the ESX-5 machinery with large conformational plasticity required to accommodate targeted protein secretion. Compared to known secretion systems, a highly dynamic state of the pore may represent a fundamental principle of bacterial secretion machineries.
External linksSci Adv / PubMed:34172453 / PubMed Central
MethodsEM (single particle) / X-ray diffraction
Resolution1.66 - 4.6 Å
Structure data

12103

7b9f

EMDB-12103, PDB-7b9f:
Structure of the mycobacterial ESX-5 Type VII Secretion System hexameric pore complex
Method: EM (single particle) / Resolution: 3.0 Å

12105

7b9s

EMDB-12105, PDB-7b9s:
Structure of the mycobacterial ESX-5 Type VII Secretion System hexameric pore complex
Method: EM (single particle) / Resolution: 3.4 Å

EMDB-12674:
Map of the hexameric ESX-5 complex from Mycobacterium xenopi with periplasmic region
Method: EM (single particle) / Resolution: 4.6 Å

PDB-7b7j:
EccD5 ubiqutin like domain from Mycobacterium xenopi
Method: X-RAY DIFFRACTION / Resolution: 1.66 Å

Chemicals

ChemComp-HOH:
WATER

Source
  • mycobacterium xenopi rivm700367 (bacteria)
  • mycobacterium xenopi (bacteria)
KeywordsMEMBRANE PROTEIN / ubiqutin-like cytosolic ESX secretion / TRANSPORT PROTEIN / Mycobacterial ESX-5 Type VII Secretion System

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