Database of articles cited by EMDB/PDB/SASBDB data

Keywords
Structure methods	All X-ray diffraction NMR electron microscopy small angle scattering neutron diffraction fiber diffraction powder diffraction infrared spectroscopy EPR fluorescence transfer theoretical model Hybrid
Author
Journal
IF	>30 >20 >10 >5 all

Title	A ferredoxin bridge connects the two arms of plant mitochondrial complex I.
Journal, issue, pages	Plant Cell, Vol. 33, Issue 6, Page 2072-2091, Year 2021
Publish date	Jul 19, 2021
Authors	Niklas Klusch / Jennifer Senkler / Özkan Yildiz / Werner Kühlbrandt / Hans-Peter Braun /
PubMed Abstract	Mitochondrial complex I is the main site for electron transfer to the respiratory chain and generates much of the proton gradient across the inner mitochondrial membrane. Complex I is composed of two ...Mitochondrial complex I is the main site for electron transfer to the respiratory chain and generates much of the proton gradient across the inner mitochondrial membrane. Complex I is composed of two arms, which form a conserved L-shape. We report the structures of the intact, 47-subunit mitochondrial complex I from Arabidopsis thaliana and the 51-subunit complex I from the green alga Polytomella sp., both at around 2.9 Å resolution. In both complexes, a heterotrimeric γ-carbonic anhydrase domain is attached to the membrane arm on the matrix side. Two states are resolved in A. thaliana complex I, with different angles between the two arms and different conformations of the ND1 (NADH dehydrogenase subunit 1) loop near the quinol binding site. The angle appears to depend on a bridge domain, which links the peripheral arm to the membrane arm and includes an unusual ferredoxin. We propose that the bridge domain participates in regulating the activity of plant complex I.
External links	Plant Cell / PubMed:33768254 / PubMed Central
Methods	EM (single particle)
Resolution	2.88 - 3.72 Å
Structure data	11872 7aqq EMDB-11872, PDB-7aqq: Cryo-EM structure of Arabidopsis thaliana Complex-I (membrane core) Method: EM (single particle) / Resolution: 3.06 Å 11873 7aqr EMDB-11873, PDB-7aqr: Cryo-EM structure of Arabidopsis thaliana Complex-I (peripheral arm) Method: EM (single particle) / Resolution: 2.91 Å 11874 7aqw EMDB-11874, PDB-7aqw: Cryo-EM structure of Arabidopsis thaliana Complex-I (membrane tip) Method: EM (single particle) / Resolution: 3.17 Å 11875 7ar7 EMDB-11875, PDB-7ar7: Cryo-EM structure of Arabidopsis thaliana complex-I (open conformation) Method: EM (single particle) / Resolution: 3.72 Å 11876 7ar8 EMDB-11876, PDB-7ar8: Cryo-EM structure of Arabidopsis thaliana complex-I (closed conformation) Method: EM (single particle) / Resolution: 3.53 Å 11877 7ar9 EMDB-11877, PDB-7ar9: Cryo-EM structure of Polytomella Complex-I (membrane arm) Method: EM (single particle) / Resolution: 2.97 Å 11878 7arb EMDB-11878, PDB-7arb: Cryo-EM structure of Arabidopsis thaliana Complex-I (complete composition) Method: EM (single particle) / Resolution: 3.41 Å 11879 7arc EMDB-11879, PDB-7arc: Cryo-EM structure of Polytomella Complex-I (peripheral arm) Method: EM (single particle) / Resolution: 2.88 Å 11880 7ard EMDB-11880, PDB-7ard: Cryo-EM structure of Polytomella Complex-I (complete composition) Method: EM (single particle) / Resolution: 3.11 Å
Chemicals	ChemComp-UQ9: Ubiquinone-9 ChemComp-PTY: PHOSPHATIDYLETHANOLAMINE / phospholipidYM ChemComp-LMN: Lauryl Maltose Neopentyl Glycol / detergentYM ChemComp-FE: Unknown entry ChemComp-PC7: (7S)-4-HYDROXY-N,N,N-TRIMETHYL-9-OXO-7-[(PALMITOYLOXY)METHYL]-3,5,8-TRIOXA-4-PHOSPHAHEXACOSAN-1-AMINIUM 4-OXIDE / phospholipidYM ChemComp-ZN: Unknown entry ChemComp-PGT: (1S)-2-{[{[(2R)-2,3-DIHYDROXYPROPYL]OXY}(HYDROXY)PHOSPHORYL]OXY}-1-[(PALMITOYLOXY)METHYL]ETHYL STEARATE / phospholipidYM ChemComp-PSF: 1,2-DICAPROYL-SN-PHOSPHATIDYL-L-SERINE ChemComp-T7X: Phosphatidylinositol ChemComp-SF4: IRON/SULFUR CLUSTER ChemComp-FES: FE2/S2 (INORGANIC) CLUSTER ChemComp-FMN: FLAVIN MONONUCLEOTIDE ChemComp-NDP: NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE ChemComp-8Q1: S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate ChemComp-CDL: CARDIOLIPIN / phospholipidYM ChemComp-HOH*: WATER
Source	arabidopsis thaliana (thale cress) Mouse-ear cress (thale cress) thale cress (thale cress) polytomella sp. pringsheim 198.80 (plant)
Keywords	ELECTRON TRANSPORT / Complex-I / Complex-I Arabidopsis