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TitleSARS-CoV-2 structure and replication characterized by in situ cryo-electron tomography.
Journal, issue, pagesNat Commun, Vol. 11, Issue 1, Page 5885, Year 2020
Publish dateNov 18, 2020
AuthorsSteffen Klein / Mirko Cortese / Sophie L Winter / Moritz Wachsmuth-Melm / Christopher J Neufeldt / Berati Cerikan / Megan L Stanifer / Steeve Boulant / Ralf Bartenschlager / Petr Chlanda /
PubMed AbstractSevere acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the causative agent of the COVID19 pandemic, is a highly pathogenic β-coronavirus. As other coronaviruses, SARS-CoV-2 is enveloped, ...Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the causative agent of the COVID19 pandemic, is a highly pathogenic β-coronavirus. As other coronaviruses, SARS-CoV-2 is enveloped, replicates in the cytoplasm and assembles at intracellular membranes. Here, we structurally characterize the viral replication compartment and report critical insights into the budding mechanism of the virus, and the structure of extracellular virions close to their native state by in situ cryo-electron tomography and subtomogram averaging. We directly visualize RNA filaments inside the double membrane vesicles, compartments associated with viral replication. The RNA filaments show a diameter consistent with double-stranded RNA and frequent branching likely representing RNA secondary structures. We report that assembled S trimers in lumenal cisternae do not alone induce membrane bending but laterally reorganize on the envelope during virion assembly. The viral ribonucleoprotein complexes (vRNPs) are accumulated at the curved membrane characteristic for budding sites suggesting that vRNP recruitment is enhanced by membrane curvature. Subtomogram averaging shows that vRNPs are distinct cylindrical assemblies. We propose that the genome is packaged around multiple separate vRNP complexes, thereby allowing incorporation of the unusually large coronavirus genome into the virion while maintaining high steric flexibility between the vRNPs.
External linksNat Commun / PubMed:33208793 / PubMed Central
MethodsEM (tomography) / EM (subtomogram averaging)
Resolution30.0 Å
Structure data

EMDB-11863:
Cryo-ET of SARS-CoV-2 infected VeroE6 cells showing budding virions.
Method: EM (tomography)

EMDB-11865:
Tomogram of SARS-CoV-2 infected and cryo-FIB milled VeroE6 cells
Method: EM (tomography)

EMDB-11866:
SARS-CoV-2 structure and replication characterized by in situ cryo-electron tomography
Method: EM (tomography)

EMDB-11867:
Cryo-ET of SARS-CoV-2 virions released from VerE6 cells
Method: EM (tomography)

EMDB-11868:
Subtomogram average of SARS-CoV-2 vRNP
Method: EM (subtomogram averaging) / Resolution: 30.0 Å

Source
  • Severe acute respiratory syndrome coronavirus 2

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