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TitleCapsid opening enables genome release of iflaviruses.
Journal, issue, pagesSci Adv, Vol. 7, Issue 1, Year 2021
Publish dateJan 1, 2021
AuthorsKarel Škubník / Lukáš Sukeník / David Buchta / Tibor Füzik / Michaela Procházková / Jana Moravcová / Lenka Šmerdová / Antonín Přidal / Robert Vácha / Pavel Plevka /
PubMed AbstractThe family Iflaviridae includes economically important viruses of the western honeybee such as deformed wing virus, slow bee paralysis virus, and sacbrood virus. Iflaviruses have nonenveloped virions ...The family Iflaviridae includes economically important viruses of the western honeybee such as deformed wing virus, slow bee paralysis virus, and sacbrood virus. Iflaviruses have nonenveloped virions and capsids organized with icosahedral symmetry. The genome release of iflaviruses can be induced in vitro by exposure to acidic pH, implying that they enter cells by endocytosis. Genome release intermediates of iflaviruses have not been structurally characterized. Here, we show that conformational changes and expansion of iflavirus RNA genomes, which are induced by acidic pH, trigger the opening of iflavirus particles. Capsids of slow bee paralysis virus and sacbrood virus crack into pieces. In contrast, capsids of deformed wing virus are more flexible and open like flowers to release their genomes. The large openings in iflavirus particles enable the fast exit of genomes from capsids, which decreases the probability of genome degradation by the RNases present in endosomes.
External linksSci Adv / PubMed:33523856 / PubMed Central
MethodsEM (single particle)
Resolution4.8 - 27.0 Å
Structure data

EMDB-11699:
Native-like genome-containing particle of DWV in acidic pH
Method: EM (single particle) / Resolution: 4.8 Å

EMDB-11703:
Open particle of the DWV containing genome in acidic pH
Method: EM (single particle) / Resolution: 27.0 Å

EMDB-11704:
Empty capsid of DWV in acidic pH
Method: EM (single particle) / Resolution: 21.0 Å

EMDB-11705:
Genome containing particle of DWV in acidic pH
Method: EM (single particle) / Resolution: 20.0 Å

EMDB-11706:
Genome containing particle of DWV in acidic pH
Method: EM (single particle) / Resolution: 25.0 Å

EMDB-11709:
Genome containing particle of DWV in acidic pH
Method: EM (single particle) / Resolution: 27.0 Å

EMDB-11711:
Genome containing particles of DWV in acidic pH
Method: EM (single particle) / Resolution: 27.0 Å

EMDB-11714:
Open empty capsid of SBV in acidic pH
Method: EM (single particle) / Resolution: 16.0 Å

EMDB-11716:
Open empty capsid of SBPV in acidic pH
Method: EM (single particle) / Resolution: 26.0 Å

11815

7al3

EMDB-11815, PDB-7al3:
Native-like genome-containing particle of DWV in acidic pH
Method: EM (single particle) / Resolution: 4.8 Å

Source
  • deformed wing virus
KeywordsVIRUS / DWV / acidic pH / genome-containing

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