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-Structure paper
Title | 3D structure of three jumbo phage heads. |
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Journal, issue, pages | J Gen Virol, Vol. 101, Issue 11, Page 1219-1226, Year 2020 |
Publish date | Aug 24, 2020 |
Authors | Emmanuelle Neumann / Takeru Kawasaki / Grégory Effantin / Leandro F Estrozi / Orawan Chatchawankanphanich / Takashi Yamada / Guy Schoehn / |
PubMed Abstract | Jumbo phages are bacteriophages that carry more than 200 kbp of DNA. In this study we characterized two jumbo phages (ΦRSL2 and ΦXacN1) and one semi-jumbo phage (ΦRP13) at the structural level by ...Jumbo phages are bacteriophages that carry more than 200 kbp of DNA. In this study we characterized two jumbo phages (ΦRSL2 and ΦXacN1) and one semi-jumbo phage (ΦRP13) at the structural level by cryo-electron microscopy. Focusing on their capsids, three-dimensional structures of the heads at resolutions ranging from 16 to 9 Å were calculated. Based on these structures we determined the geometrical basis on which the icosahedral capsids of these phages are constructed, which includes the accessory and decorative proteins that complement them. A triangulation number novel to (ΦRP13; =21) was discovered as well as two others, which are more common for jumbo phages (=27 and =28). Based on one of the structures we also provide evidence that accessory or decorative proteins are not a prerequisite for maintaining the structural integrity of very large capsids. |
External links | J Gen Virol / PubMed:32840476 |
Methods | EM (single particle) |
Resolution | 9.0 - 16.0 Å |
Structure data | EMDB-11178: EMDB-11179: EMDB-11180: |