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-Structure paper
Title | In-cell architecture of an actively transcribing-translating expressome. |
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Journal, issue, pages | Science, Vol. 369, Issue 6503, Page 554-557, Year 2020 |
Publish date | Jul 31, 2020 |
![]() | Francis J O'Reilly / Liang Xue / Andrea Graziadei / Ludwig Sinn / Swantje Lenz / Dimitry Tegunov / Cedric Blötz / Neil Singh / Wim J H Hagen / Patrick Cramer / Jörg Stülke / Julia Mahamid / Juri Rappsilber / ![]() ![]() |
PubMed Abstract | Structural biology studies performed inside cells can capture molecular machines in action within their native context. In this work, we developed an integrative in-cell structural approach using the ...Structural biology studies performed inside cells can capture molecular machines in action within their native context. In this work, we developed an integrative in-cell structural approach using the genome-reduced human pathogen We combined whole-cell cross-linking mass spectrometry, cellular cryo-electron tomography, and integrative modeling to determine an in-cell architecture of a transcribing and translating expressome at subnanometer resolution. The expressome comprises RNA polymerase (RNAP), the ribosome, and the transcription elongation factors NusG and NusA. We pinpointed NusA at the interface between a NusG-bound elongating RNAP and the ribosome and propose that it can mediate transcription-translation coupling. Translation inhibition dissociated the expressome, whereas transcription inhibition stalled and rearranged it. Thus, the active expressome architecture requires both translation and transcription elongation within the cell. |
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Methods | EM (subtomogram averaging) |
Resolution | 5.6 - 9.7 Å |
Structure data | ![]() EMDB-10677: ![]() EMDB-10678: ![]() EMDB-10679: ![]() EMDB-10680: ![]() EMDB-10681: ![]() EMDB-10682: ![]() EMDB-10683: ![]() EMDB-10684: ![]() EMDB-10685: ![]() EMDB-10686: ![]() EMDB-10687: |
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