+Search query
-Structure paper
Title | Cryo-EM-On-a-Chip: Custom-Designed Substrates for the 3D Analysis of Macromolecules. |
---|---|
Journal, issue, pages | Small, Vol. 15, Issue 21, Page e1900918, Year 2019 |
Publish date | Apr 8, 2019 |
![]() | Nick A Alden / A Cameron Varano / William J Dearnaley / Maria J Solares / William Y Luqiu / Yanping Liang / Zhi Sheng / Sarah M McDonald / John Damiano / Jennifer McConnell / Madeline J Dukes / Deborah F Kelly / ![]() |
PubMed Abstract | The fight against human disease requires a multidisciplinary scientific approach. Applying tools from seemingly unrelated areas, such as materials science and molecular biology, researchers can ...The fight against human disease requires a multidisciplinary scientific approach. Applying tools from seemingly unrelated areas, such as materials science and molecular biology, researchers can overcome long-standing challenges to improve knowledge of molecular pathologies. Here, custom-designed substrates composed of silicon nitride (SiN) are used to study the 3D attributes of tumor suppressor proteins that function in DNA repair events. New on-chip preparation strategies enable the isolation of native protein complexes from human cancer cells. Combined techniques of cryo-electron microscopy (EM) and molecular modeling reveal a new modified form of the p53 tumor suppressor present in aggressive glioblastoma multiforme cancer cells. Taken together, the findings provide a radical new design for cryo-EM substrates to evaluate the structures of disease-related macromolecules. |
![]() | ![]() ![]() ![]() |
Methods | EM (single particle) |
Resolution | 10.0 Å |
Structure data | ![]() EMDB-0378: |
Source |
|