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TitleStructural reorganization of the chromatin remodeling enzyme Chd1 upon engagement with nucleosomes.
Journal, issue, pagesElife, Vol. 6, Year 2017
Publish dateMar 23, 2017
AuthorsRamasubramanian Sundaramoorthy / Amanda L Hughes / Vijender Singh / Nicola Wiechens / Daniel P Ryan / Hassane El-Mkami / Maxim Petoukhov / Dmitri I Svergun / Barbara Treutlein / Salina Quack / Monika Fischer / Jens Michaelis / Bettina Böttcher / David G Norman / Tom Owen-Hughes /
PubMed AbstractThe yeast Chd1 protein acts to position nucleosomes across genomes. Here, we model the structure of the Chd1 protein in solution and when bound to nucleosomes. In the apo state, the DNA-binding ...The yeast Chd1 protein acts to position nucleosomes across genomes. Here, we model the structure of the Chd1 protein in solution and when bound to nucleosomes. In the apo state, the DNA-binding domain contacts the edge of the nucleosome while in the presence of the non-hydrolyzable ATP analog, ADP-beryllium fluoride, we observe additional interactions between the ATPase domain and the adjacent DNA gyre 1.5 helical turns from the dyad axis of symmetry. Binding in this conformation involves unravelling the outer turn of nucleosomal DNA and requires substantial reorientation of the DNA-binding domain with respect to the ATPase domains. The orientation of the DNA-binding domain is mediated by sequences in the N-terminus and mutations to this part of the protein have positive and negative effects on Chd1 activity. These observations indicate that the unfavorable alignment of C-terminal DNA-binding region in solution contributes to an auto-inhibited state.
External linksElife / PubMed:28332978 / PubMed Central
MethodsSAS (X-ray synchrotron) / EM (single particle)
Resolution15.0 - 20.0 Å
Structure data

SASDBU7:
DNA binding domain (DBD) of chromo domain-containing protein 1 (Chd1: 1009-1274)
Method: SAXS/SANS

SASDBV7:
Chromo-ATPase-DBD domains of chromo domain-containing protein 1 (Chd1: 133-1305)
Method: SAXS/SANS

SASDBW7:
N-terminal and chromo-ATPase-DBD domains of chromo domain-containing protein 1 (Chd1: 1-1305)
Method: SAXS/SANS

SASDBX7:
Chromo-ATPase domains of chromo domain-containing protein 1 (Chd1: 133-1010)
Method: SAXS/SANS

SASDBY7:
N-terminal and chromo-ATPase domains of chromo domain-containing protein 1 (Chd1: 1-1010)
Method: SAXS/SANS

EMDB-3502:
Structural reorganization of the chromatin remodeling enzyme Chd1 upon engagement with nucleosomes
Method: EM (single particle) / Resolution: 15.0 Å

EMDB-3517:
Structural reorganization of the chromatin remodeling enzyme Chd1 upon engagement with nucleosomes.
Method: EM (single particle) / Resolution: 20.0 Å

Source
  • Saccharomyces cerevisiae (brewer's yeast)
  • Xenopus laevis (African clawed frog)

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