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TitlePreparing monodisperse macromolecular samples for successful biological small-angle X-ray and neutron-scattering experiments.
Journal, issue, pagesNat Protoc, Vol. 11, Issue 11, Page 2122-2153, Year 2016
Publish dateOct 6, 2016
AuthorsCy M Jeffries / Melissa A Graewert / Clément E Blanchet / David B Langley / Andrew E Whitten / Dmitri I Svergun /
PubMed AbstractSmall-angle X-ray scattering (SAXS) and small-angle neutron scattering (SANS) are techniques used to extract structural parameters and determine the overall structures and shapes of biological ...Small-angle X-ray scattering (SAXS) and small-angle neutron scattering (SANS) are techniques used to extract structural parameters and determine the overall structures and shapes of biological macromolecules, complexes and assemblies in solution. The scattering intensities measured from a sample contain contributions from all atoms within the illuminated sample volume, including the solvent and buffer components, as well as the macromolecules of interest. To obtain structural information, it is essential to prepare an exactly matched solvent blank so that background scattering contributions can be accurately subtracted from the sample scattering to obtain the net scattering from the macromolecules in the sample. In addition, sample heterogeneity caused by contaminants, aggregates, mismatched solvents, radiation damage or other factors can severely influence and complicate data analysis, so it is essential that the samples be pure and monodisperse for the duration of the experiment. This protocol outlines the basic physics of SAXS and SANS, and it reveals how the underlying conceptual principles of the techniques ultimately 'translate' into practical laboratory guidance for the production of samples of sufficiently high quality for scattering experiments. The procedure describes how to prepare and characterize protein and nucleic acid samples for both SAXS and SANS using gel electrophoresis, size-exclusion chromatography (SEC) and light scattering. Also included are procedures that are specific to X-rays (in-line SEC-SAXS) and neutrons, specifically preparing samples for contrast matching or variation experiments and deuterium labeling of proteins.
External linksNat Protoc / PubMed:27711050 / PubMed Central
MethodsSAS (X-ray synchrotron)
Structure data

SASDBJ3:
Bovine serum albumin, monomer from SEC-SAXS (Bovine serum albumin, monomer, BSA monomer)
Method: SAXS/SANS

SASDBK3:
Bovine serum albumin, dimer from SEC-SAXS (Bovine serum albumin, dimer, BSA dimer)
Method: SAXS/SANS

Source
  • Bos taurus (cattle)

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