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Structure paper

TitleAn improved fluorescent protein tag and its nanobodies for membrane protein expression, stability assay, and purification
Journal, issue, pagesTo Be Published
Publish dateSep 6, 2020 (structure data deposition date)
AuthorsCai, H. / Yao, H. / Li, T. / Hutter, C. / Tang, Y. / Li, Y. / Seeger, M. / Li, D.
External linksSearch PubMed
MethodsX-ray diffraction
Resolution2.77 Å
Structure data

PDB-7cz0:
Crystal structure of a thermostable green fluorescent protein (TGP) with a synthetic nanobody (Sb92)
Method: X-RAY DIFFRACTION / Resolution: 2.77 Å

Chemicals

ChemComp-GOL:
GLYCEROL

ChemComp-CAD:
CACODYLIC ACID

ChemComp-ACT:
ACETATE ION

ChemComp-CAC:
CACODYLATE ION

Source
  • galaxea fascicularis (invertebrata)
  • synthetic construct (others)
KeywordsFLUORESCENT PROTEIN / complex / GFP / nanobody / single-chain antibody / sybody / synthetic antibody / TGP / thermostable green fluorescent protein

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