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TitleStructure of Escherichia coli DNA gyrase with chirally wrapped DNA supports ratchet-and-pawl mechanism for an ATP-powered supercoiling motor
Journal, issue, pagesProceedings of the National Academy of Sciences USA, Year 2024
Publish dateJul 30, 2024 (structure data deposition date)
AuthorsMichalczyk E / Pakosz-Stepien Z / Liston J / Gittins O / Pabis M / Heddle JG / Ghilarov D
External linksSearch PubMed
MethodsEM (single particle)
Resolution2.3 - 3.22 Å
Structure data

EMDB-51211: Gyrase holocomplex with chirally wrapped DNA: a focussed map for the CTD II
Method: EM (single particle) / Resolution: 2.94 Å

EMDB-51212: Gyrase holocomplex with chirally wrapped DNA: a focussed map for the CTD I
Method: EM (single particle) / Resolution: 3.22 Å

EMDB-51213: Gyrase holocomplex with chirally wrapped DNA: a focussed map for the CTD II & T-DNA
Method: EM (single particle) / Resolution: 3.14 Å

EMDB-51215: Gyrase holocomplex with chirally wrapped DNA: a focussed map for the GHKL domain (chain B)
Method: EM (single particle) / Resolution: 2.93 Å

EMDB-51216: Gyrase holocomplex with chirally wrapped DNA: a focussed map for the GHKL domain (chain D)
Method: EM (single particle) / Resolution: 2.92 Å

EMDB-51218: E.coli gyrase holocomplex with 217 bp chirally wrapped DNA (consensus map)
Method: EM (single particle) / Resolution: 2.3 Å

51222

9gbv

EMDB-51222, PDB-9gbv:
E.coli gyrase holocomplex with chirally wrapped 217 bp DNA fragment
Method: EM (single particle) / Resolution: 2.32 Å

Chemicals

ChemComp-MG:
Unknown entry

Source
  • escherichia coli (E. coli)
  • escherichia phage mu (virus)
KeywordsISOMERASE / DNA gyrase / type II topoisomerase / supercoiling / DNA crossover

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