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Structure paper

TitleStructural basis of target recognition by the DNA binding domain of McrBC
Journal, issue, pagesTo Be Published
Publish dateFeb 27, 2023 (structure data deposition date)
AuthorsAdhav, V.A. / Saikrishnan, K.
External linksSearch PubMed
MethodsX-ray diffraction
Resolution1.55 - 2.1 Å
Structure data

PDB-8ijo:
Structure of DNA binding domain of McrBC endonuclease bound to DNA: Y41F-L68F double mutant
Method: X-RAY DIFFRACTION / Resolution: 1.65 Å

PDB-8ijp:
Structure of DNA binding domain of McrBC endonuclease bound to DNA: L68Y mutant
Method: X-RAY DIFFRACTION / Resolution: 1.55 Å

PDB-8ik4:
Structure of DNA binding domain of McrBC endonuclease bound to hemimethylated DNA: L68F mutant
Method: X-RAY DIFFRACTION / Resolution: 2.1 Å

PDB-8ik8:
Structure of DNA binding domain of McrBC endonuclease bound to DNA: L68F mutant
Method: X-RAY DIFFRACTION / Resolution: 1.8 Å

PDB-8ikd:
Structure of DNA binding domain of McrBC endonuclease bound to DNA: Y41F-L68Y double mutant
Method: X-RAY DIFFRACTION / Resolution: 2.1 Å

Chemicals

ChemComp-BTB:
2-[BIS-(2-HYDROXY-ETHYL)-AMINO]-2-HYDROXYMETHYL-PROPANE-1,3-DIOL / pH buffer*YM

ChemComp-HOH:
WATER

Source
  • escherichia coli k-12 (bacteria)
  • dna molecule (others)
KeywordsDNA BINDING PROTEIN / McrB-NTD / 5-methylcytosine / Restriction endonucleases / restriction endonuclease

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