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TitleStructural insights into the augmin-microtubule interaction reveal a conserved function of the HAUS6 CH domain in orienting augmin on microtubules
Journal, issue, pagesTo Be Published, Year 2025
Publish dateFeb 14, 2025 (structure data deposition date)
AuthorsWuertz M / Tonon G / Vermeulen BJA / Zezlina M / Gao G / Annett N / Seidl A / Koenig M / Harkenthal M / Eustermann S ...Wuertz M / Tonon G / Vermeulen BJA / Zezlina M / Gao G / Annett N / Seidl A / Koenig M / Harkenthal M / Eustermann S / Erhardt S / Lolicato F / Schiebel E / Pfeffer S
External linksTo Be Published / Search PubMed
MethodsEM (helical sym.) / EM (single particle)
Resolution3.9 - 4.9 Å
Structure data

EMDB-52832: Helical reconstruction of a D. melanogaster N-clamp-decorated microtubule
Method: EM (helical sym.) / Resolution: 4.0 Å

54160

9rpd

EMDB-54160: D. melanogaster augmin TII N-clamp (GST-fusion) bound to a microtubule
PDB-9rpd: D. melanogaster Augmin TII N-clamp (GST-fusion) bound to a microtubule, well-defined subset of particles
Method: EM (single particle) / Resolution: 3.9 Å

54161

9rpd

EMDB-54161, PDB-9rpd:
D. melanogaster Augmin TII N-clamp (GST-fusion) bound to a microtubule, well-defined subset of particles
Method: EM (single particle) / Resolution: 4.9 Å

EMDB-54174: D. melanogaster Augmin TII N-clamp bound to a microtubule
Method: EM (single particle) / Resolution: 4.7 Å

Source
  • drosophila melanogaster (fruit fly)
  • sus scrofa (pig)
  • schistosoma japonicum (invertebrata)
  • aequorea victoria (jellyfish)
KeywordsCELL CYCLE / augmin / N-clamp / TII / microtubule / branching / nucleation / HAUS / HAUS augmin-like complex subunit / HAUS6 / HAUS7 / HAUS2 / HAUS8 / Dgt6 / Msd5 / Dgt4 / Msd1 / Drosophila melanogaster / complex / CH domain / calponin homology domain / MAP / cell division

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