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Structure paper

TitleUnexpected enzyme-catalysed [4+2] cycloaddition and rearrangement in polyether antibiotic biosynthesis
Journal, issue, pagesNat Catal, Year 2019
Publish dateJan 15, 2019 (structure data deposition date)
AuthorsLittle, R. / Paiva, F.C.R. / Jenkins, R. / Hong, H. / Sun, Y. / Demydchuk, Y. / Samborskyy, M. / Tosin, M. / Leeper, F.J. / Dias, M.V.B. / Leadlay, P.F.
External linksNat Catal / Search PubMed
MethodsX-ray diffraction
Resolution1.7 - 1.8 Å
Structure data

PDB-6nnw:
Tsn15 in complex with substrate intermediate
Method: X-RAY DIFFRACTION / Resolution: 1.7 Å

PDB-6noi:
Crystal structure of Tsn15 in apo form
Method: X-RAY DIFFRACTION / Resolution: 1.8 Å

Chemicals

ChemComp-KUJ:
(3E)-3-{(1S,4S,4aS,5R,8aS)-1-[(2E,4R,7S,8E,10S)-1,7-dihydroxy-10-{(2R,3S,5R)-5-[(1S)-1-methoxyethyl]-3-methyloxolan-2-yl}-4-methylundeca-2,8-dien-2-yl]-4,5-dimethyloctahydro-3H-2-benzopyran-3-ylidene}oxolane-2,4-dione

ChemComp-PO4:
PHOSPHATE ION

ChemComp-PPI:
PROPANOIC ACID

ChemComp-HOH:
WATER

ChemComp-PGE:
TRIETHYLENE GLYCOL

ChemComp-PEG:
DI(HYDROXYETHYL)ETHER

Source
  • streptomyces longisporoflavus (bacteria)
KeywordsUNKNOWN FUNCTION / Complex / cyclase / LYASE / tetronasin

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