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Structure paper

TitleStructural Insights into a Side Chain Cross-Linking Biarylitide P450 from RiPP Biosynthesis
Journal, issue, pagesAcs Catalysis, Page 812-826, Year 2024
Publish dateSep 6, 2023 (structure data deposition date)
AuthorsHansen, M.H. / Keto, A. / Treisman, M. / Sasi, V.M. / Coe, L. / Zhao, Y. / Padva, L. / Hess, C. / Leichthammer, V. / Machell, D.L. ...Hansen, M.H. / Keto, A. / Treisman, M. / Sasi, V.M. / Coe, L. / Zhao, Y. / Padva, L. / Hess, C. / Leichthammer, V. / Machell, D.L. / Schittenhelm, R.B. / Jackson, C.J. / Tailhades, J. / Crusemann, M. / De Voss, J.J. / Krenske, E.H. / Cryle, M.J.
External linksAcs Catalysis / Search PubMed
MethodsX-ray diffraction
Resolution1.79 - 2.15 Å
Structure data

PDB-8u2m:
Structure of P450Blt from Micromonospora sp. MW-13 in Complex with Biarylitide
Method: X-RAY DIFFRACTION / Resolution: 1.79 Å

PDB-8u3n:
Structure of P450Blt from Micromonospora sp. MW-13
Method: X-RAY DIFFRACTION / Resolution: 2.15 Å

PDB-8ukz:
Structure of P450Blt from Micromonospora sp. MW-13 with E238A Mutation
Method: X-RAY DIFFRACTION / Resolution: 1.95 Å

Chemicals

ChemComp-ACT:
ACETATE ION

ChemComp-PG4:
TETRAETHYLENE GLYCOL / precipitant*YM

ChemComp-HEM:
PROTOPORPHYRIN IX CONTAINING FE

ChemComp-NA:
Unknown entry

ChemComp-TOE:
2-[2-(2-METHOXY-ETHOXY)-ETHOXY]-ETHOXYL

ChemComp-MRY:
MESO-ERYTHRITOL

ChemComp-TFA:
trifluoroacetic acid

ChemComp-HOH:
WATER

ChemComp-BGC:
beta-D-glucopyranose

ChemComp-K:
Unknown entry

ChemComp-SCN:
THIOCYANATE ION

ChemComp-PEG:
DI(HYDROXYETHYL)ETHER

Source
  • micromonospora sp. mw-13 (bacteria)
  • synthetic construct (others)
KeywordsOXIDOREDUCTASE / complex / biarylitide / crosslink / mutant

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