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Structure paper

TitleMyeloperoxidase transforms chromatin into Neutrophil Extracellular Traps
Journal, issue, pagesTo Be Published
Publish dateAug 7, 2024 (structure data deposition date)
AuthorsBurn GL / Raisch T / Tacke S / Winkler M / Prumbaum D / Thee S / Raunser S / Zychlinsky A
External linksSearch PubMed
MethodsEM (single particle)
Resolution2.79 - 3.99 Å
Structure data

EMDB-51295, PDB-9gen:
Recombinant Myeloperoxidase bound to nucleosome core particle
Method: EM (single particle) / Resolution: 3.76 Å

EMDB-51296, PDB-9geo:
Nucleosome core particle
Method: EM (single particle) / Resolution: 2.79 Å

EMDB-51297, PDB-9gep:
Native monomeric Myeloperoxidase bound to nucleosome core particle
Method: EM (single particle) / Resolution: 2.89 Å

EMDB-51298: Native dimeric Myeloperoxidase bound to nucleosome core particle; nucleosome focused map
Method: EM (single particle) / Resolution: 3.01 Å

EMDB-51299: Native dimeric Myeloperoxidase bound to nucleosome core particle; MPO focused map
Method: EM (single particle) / Resolution: 2.98 Å

EMDB-51300: Native dimeric Myeloperoxidase bound to nucleosome core particle; consensus map
Method: EM (single particle) / Resolution: 3.12 Å

EMDB-51301, PDB-9geq:
Native dimeric Myeloperoxidase bound to nucleosome core particle; composite map
Method: EM (single particle) / Resolution: 3.12 Å

EMDB-51302: Native dimeric Myeloperoxidase bound to nucleosome core particle, intermediate state, nucleosome focused map
Method: EM (single particle) / Resolution: 3.52 Å

EMDB-51303: Native dimeric Myeloperoxidase bound to nucleosome core particle, intermediate state, map focused on MPO
Method: EM (single particle) / Resolution: 3.87 Å

EMDB-51304: Native dimeric Myeloperoxidase bound to nucleosome core particle, intermediate state, consensus map
Method: EM (single particle) / Resolution: 3.58 Å

EMDB-51305, PDB-9ger:
Native dimeric Myeloperoxidase bound to nucleosome core particle, intermediate state; composite map
Method: EM (single particle) / Resolution: 3.58 Å

EMDB-51306: Native monomeric Myeloperoxidase bound to nucleosome core particle, late time point
Method: EM (single particle) / Resolution: 3.99 Å

EMDB-52865, PDB-9ihd:
Nucleosome core particle bound by one molecule of DTT-reduced native monomeric myeloperoxidase
Method: EM (single particle) / Resolution: 2.97 Å

EMDB-52866, PDB-9ihe:
Nucleosome core particle bound by two molecules of DTT-reduced native monomeric myeloperoxidase
Method: EM (single particle) / Resolution: 2.95 Å

EMDB-52867: Nucleosome core particle bound by one monomer and one dimer of of DTT-reduced native myeloperoxidase; map focused on nucleosome/MPO monomer
Method: EM (single particle) / Resolution: 3.13 Å

EMDB-52868: Nucleosome core particle bound by one monomer and one dimer of of DTT-reduced native myeloperoxidase; map focused on MPO dimer
Method: EM (single particle) / Resolution: 3.17 Å

EMDB-52869: Nucleosome core particle bound by one monomer and one dimer of of DTT-reduced native myeloperoxidase; consensus map
Method: EM (single particle) / Resolution: 3.16 Å

EMDB-52870, PDB-9ihf:
Nucleosome core particle bound by one monomer and one dimer of of DTT-reduced native myeloperoxidase
Method: EM (single particle) / Resolution: 3.16 Å

Chemicals

ChemComp-NAG:
2-acetamido-2-deoxy-beta-D-glucopyranose

ChemComp-HOH:
WATER

ChemComp-HEM:
PROTOPORPHYRIN IX CONTAINING FE

ChemComp-CL:
Unknown entry

ChemComp-CA:
Unknown entry

Source
  • homo sapiens (human)
  • xenopus laevis (African clawed frog)
  • synthetic construct (others)
KeywordsIMMUNE SYSTEM / Peroxidase / innate immunity / histone / acidic patch / NUCLEAR PROTEIN / NETs

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