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TitleBiochemical characterization of a novel Trypanosoma brucei glycosomal isocitrate dehydrogenase with dual coenzyme specificity (NADP+/NAD+)
Journal, issue, pagesTo Be Published
Publish dateAug 27, 2018 (structure data deposition date)
AuthorsWang, X. / Inaoka, D.K. / Shiba, T. / Balogun, E.O. / Ziebart, N. / Allman, S. / Watanabe, Y. / Nozaki, T. / Boshart, M. / Bringaud, F. ...Wang, X. / Inaoka, D.K. / Shiba, T. / Balogun, E.O. / Ziebart, N. / Allman, S. / Watanabe, Y. / Nozaki, T. / Boshart, M. / Bringaud, F. / Harada, S. / Kita, K.
External linksSearch PubMed
MethodsX-ray diffraction
Resolution2.4 - 3.2 Å
Structure data

PDB-6aj6:
Crystal structure of Trypanosoma brucei glycosomal isocitrate dehydrogenase in complex with NADP+
Method: X-RAY DIFFRACTION / Resolution: 3.2 Å

PDB-6aj8:
Crystal structure of Trypanosoma brucei glycosomal isocitrate dehydrogenase in complex with NADP+, alpha-ketoglutarate and ca2+
Method: X-RAY DIFFRACTION / Resolution: 2.4 Å

PDB-6aja:
Crystal structure of Trypanosoma brucei glycosomal isocitrate dehydrogenase in complex with NADPH, alpha-ketoglutarate and ca2+
Method: X-RAY DIFFRACTION / Resolution: 2.85 Å

PDB-6ajb:
Crystal structure of Trypanosoma brucei glycosomal isocitrate dehydrogenase in complex with NADH, alpha-ketoglutarate and ca2+
Method: X-RAY DIFFRACTION / Resolution: 2.9 Å

PDB-6ajc:
Crystal structure of Trypanosoma cruzi cytosolic isocitrate dehydrogenase in complex with NADP+, isocitrate and ca2+
Method: X-RAY DIFFRACTION / Resolution: 2.4 Å

Chemicals

ChemComp-NAP:
NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE

ChemComp-CA:
Unknown entry

ChemComp-AKG:
2-OXOGLUTARIC ACID

ChemComp-HOH:
WATER

ChemComp-GOL:
GLYCEROL

ChemComp-NAD:
NICOTINAMIDE-ADENINE-DINUCLEOTIDE / NAD*YM

ChemComp-ICT:
ISOCITRIC ACID

Source
  • trypanosoma brucei brucei (eukaryote)
  • trypanosoma cruzi (strain cl brener) (eukaryote)
KeywordsOXIDOREDUCTASE / Trypanosoma brucei / glycosome / metabolism / isocitrate dehydrogenase / Trypanosoma cruzi

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