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TitleIntersubunit Coupling Enables Fast CO2-Fixation by Reductive Carboxylases
Journal, issue, pagesAcs Cent. Sci., Year 2022
Publish dateDec 5, 2018 (structure data deposition date)
AuthorsDeMirci, H. / Rao, Y. / Stoffel, G.M. / Vogeli, B. / Schell, K. / Gomez, A. / Batyuk, A. / Gati, C. / Sierra, R.G. / Hunter, M.S. ...DeMirci, H. / Rao, Y. / Stoffel, G.M. / Vogeli, B. / Schell, K. / Gomez, A. / Batyuk, A. / Gati, C. / Sierra, R.G. / Hunter, M.S. / Dao, E.H. / Ciftci, H.I. / Hayes, B. / Poitevin, F. / Li, P.N. / Kaur, M. / Tono, K. / Saez, D.A. / Deutsch, S. / Yoshikuni, Y. / Grubmuller, H. / Erb, T.J. / Vohringer-Martinez, E. / Wakatsuki, S.
External linksAcs Cent. Sci. / Search PubMed
MethodsX-ray diffraction
Resolution1.722 - 2.1 Å
Structure data

PDB-6na3:
Crystal Structure of Apo-form of ECR
Method: X-RAY DIFFRACTION / Resolution: 1.8 Å

PDB-6na4:
Co crystal structure of ECR with Butryl-CoA
Method: X-RAY DIFFRACTION / Resolution: 1.722 Å

PDB-6na5:
Crystal Structure of ECR in complex with NADP+
Method: X-RAY DIFFRACTION / Resolution: 1.75 Å

PDB-6na6:
Serial Femtosecond X-ray Crystallography Structure of ECR in complex with NADPH
Method: X-RAY DIFFRACTION / Resolution: 2.1 Å

Chemicals

ChemComp-VES:
Pyrrolidine

ChemComp-CL:
Unknown entry

ChemComp-HOH:
WATER

ChemComp-NAP:
NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE

ChemComp-2EC:
9-ETHYL-9H-PURIN-6-YLAMINE

ChemComp-YAS:
~{S}-[2-[3-[[(2~{R})-4-[[[(2~{S},3~{R},4~{S},5~{R})-5-(6-aminopurin-9-yl)-4-oxidanyl-3-phosphonooxy-oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-3,3-dimethyl-2-oxidanyl-butanoyl]amino]propanoylamino]ethyl] butanethioate

ChemComp-NDP:
NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE

Source
  • kitasatospora setae (strain atcc 33774 / dsm 43861 / jcm 3304 / kcc a-0304 / nbrc 14216 / km-6054) (bacteria)
KeywordsOXIDOREDUCTASE / C-cycling ECR / C-cycling ECR Butryl-CoA / ECR C-cycling NADP+ / C-cycling / ECR / NADPH / SFX Ambient temperature

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