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TitleRecruitment of a Buried K+ Ion to Stabilize the Negative Charge of Ionized P1 in the Hydrophobic Pocket: Crystal Structures of Glu18, Gln18, Asp18 and Asn18 Variants of Turkey Ovomucoid Inhibitor Third Domain Complexed with Streptomyces griseus Protease B at Various pHs
Journal, issue, pagesTo be Published
Publish dateMar 25, 1999 (structure data deposition date)
AuthorsHuang, K. / Lu, W. / Anderson, S. / Laskowski Jr., M. / James, M.N.G.
External linksSearch PubMed
MethodsX-ray diffraction
Resolution1.75 - 1.8 Å
Structure data

PDB-1sge:
GLU 18 VARIANT OF TURKEY OVOMUCOID INHIBITOR THIRD DOMAIN COMPLEXED WITH STREPTOMYCES GRISEUS PROTEINASE B AT PH 6.5
Method: X-RAY DIFFRACTION / Resolution: 1.8 Å

PDB-1sgn:
ASN 18 VARIANT OF TURKEY OVOMUCOID INHIBITOR THIRD DOMAIN COMPLEXED WITH STREPTOMYCES GRISEUS PROTEINASE B
Method: X-RAY DIFFRACTION / Resolution: 1.8 Å

PDB-2sgd:
ASP 18 VARIANT OF TURKEY OVOMUCOID INHIBITOR THIRD DOMAIN COMPLEXED WITH STREPTOMYCES GRISEUS PROTEINASE B AT PH 10.7
Method: X-RAY DIFFRACTION / Resolution: 1.8 Å

PDB-2sgf:
PHE 18 VARIANT OF TURKEY OVOMUCOID INHIBITOR THIRD DOMAIN COMPLEXED WITH STREPTOMYCES GRISEUS PROTEINASE B
Method: X-RAY DIFFRACTION / Resolution: 1.75 Å

Chemicals

ChemComp-PO4:
PHOSPHATE ION

ChemComp-HOH:
WATER

ChemComp-K:
Unknown entry

Source
  • meleagris gallopavo (turkey)
  • streptomyces griseus (bacteria)
KeywordsHYDROLASE/HYDROLASE INHIBITOR / COMPLEX (SERINE PROTEASE-INHIBITOR) / SERINE PROTEINASE / PROTEIN INHIBITOR / HYDROLASE-HYDROLASE INHIBITOR COMPLEX

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