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TitleStructural Basis of Increased Resistance to Thermal Denaturation Induced by Single Amino Acid Substitution in the Sequence of Beta-Glucosidase a from Bacillus Polymyxa.
Journal, issue, pagesProteins: Struct. ,Funct. , Genet., Vol. 33, Page 567-576, Year 1998
Publish dateJul 6, 2000 (structure data deposition date)
AuthorsSanz-Aparicio, J. / Hermoso, J.A. / Martinez-Ripoll, M. / Gonzalez, B. / Lopez-Camacho, C. / Polaina, J.
External linksProteins: Struct. ,Funct. , Genet. / PubMed:9849940
MethodsX-ray diffraction
Resolution2 Å
Structure data

PDB-1e4i:
2-deoxy-2-fluoro-beta-D-glucosyl/enzyme intermediate complex of the beta-glucosidase from Bacillus polymyxa
Method: X-RAY DIFFRACTION / Resolution: 2 Å

Chemicals

ChemComp-G2F:
2-deoxy-2-fluoro-alpha-D-glucopyranose

ChemComp-NFG:
2,4-dinitrophenyl 2-deoxy-2-fluoro-beta-D-glucopyranoside

ChemComp-HOH:
WATER

Source
  • bacillus polymyxa (bacteria)
KeywordsHYDROLASE / FAMILY 1 GLYCOSYL HYDROLASE / COVALENT ENZYME-GLYCOSIDE INTERMEDIATE / ALPHA/BETA BARREL

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