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Structure paper

TitleCrystal structure of the family 7 endoglucanase I (Cel7B) from Humicola insolens at 2.2 A resolution and identification of the catalytic nucleophile by trapping of the covalent glycosyl-enzyme intermediate.
Journal, issue, pagesBiochem. J., Vol. 335 ( Pt 2), Page 409-416, Year 1998
Publish dateFeb 3, 2000 (structure data deposition date)
AuthorsMacKenzie, L.F. / Sulzenbacher, G. / Divne, C. / Jones, T.A. / Woldike, H.F. / Schulein, M. / Withers, S.G. / Davies, G.J.
External linksBiochem. J. / PubMed:9761741
MethodsX-ray diffraction
Resolution1.75 - 2.2 Å
Structure data

PDB-1dym:
Humicola insolens Endocellulase Cel7B (EG 1) E197A Mutant
Method: X-RAY DIFFRACTION / Resolution: 1.75 Å

PDB-2a39:
HUMICOLA INSOLENS ENDOCELLULASE EGI NATIVE STRUCTURE
Method: X-RAY DIFFRACTION / Resolution: 2.2 Å

Chemicals

ChemComp-NAG:
2-acetamido-2-deoxy-beta-D-glucopyranose

ChemComp-HOH:
WATER

Source
  • humicola insolens (fungus)
KeywordsHYDROLASE / ENDOGLUCANASE / CELLULOSE DEGRADATION / GLYCOSIDASE / GLYCOPROTEIN / GLYCOSYNTHASE / GLYCOSIDE HYDROLASE FAMILY 7 / CELLULASE / GLYCOSYLATED PROTEIN

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