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TitleMetabolite glues as a means of purine sensing and chemotherapeutic response.
Journal, issue, pagesNature, Vol. 655, Issue 8125, Page 1300-1308, Year 2026
Publish dateJul 15, 2026
AuthorsSamuel R Witus / Megan M Kober / Heegwang Roh / Zhi Yang / Fouad Choueiry / Avani S Ghate / Denis V Titov / Michael Rapé /
PubMed AbstractMolecular glues stabilize weak interactions to impart new functionalities to complexes. Although molecular glues have been described in plant signalling and as human therapeutics, it is unclear ...Molecular glues stabilize weak interactions to impart new functionalities to complexes. Although molecular glues have been described in plant signalling and as human therapeutics, it is unclear whether this modality provides endogenous regulation in human cells. Here we show that purine nucleotides are molecular glues that tether the rate-limiting enzyme in purine biosynthesis-phosphoribosyl pyrophosphate amidotransferase (PPAT)-to its inhibitor NUDT5. This mechanism allows cells to sense the levels of purines and to establish essential feedback control of their synthesis. We refer to such molecules as metabolite glues. Thiopurine chemotherapeutics, which have been in clinical use since the 1950s, glue the same complex but adopt distinct orientations for enhanced function. Unlike most known glues, the PPAT-NUDT5 metabolite-glue pocket can adjust its conformation to notable compound alterations, enabling increased glue potency and improved on-target activity. We therefore identify endogenous metabolite glues as a mode of nutrient sensing that can be exploited for therapeutic benefit.
External linksNature / PubMed:42457960 / PubMed Central
MethodsEM (single particle)
Resolution2.8 - 3.1 Å
Structure data

72099

9q0m

EMDB-72099, PDB-9q0m:
Cryo-EM structure of PPAT-NUDT5 complex bound to adenosine-5'-monophosphate (AMP)
Method: EM (single particle) / Resolution: 3.1 Å

72100

9q0n

EMDB-72100, PDB-9q0n:
Cryo-EM structure of PPAT-NUDT5 complex bound to 6-methylthioinosine-5'-monophosphate (6-meTIMP)
Method: EM (single particle) / Resolution: 2.8 Å

72101

9q0o

EMDB-72101, PDB-9q0o:
Cryo-EM structure of PPAT-NUDT5 complex bound to 6-benzylthioinosine-5'-monophosphate (6-benzylTIMP)
Method: EM (single particle) / Resolution: 2.9 Å

Chemicals

ChemComp-SF4:
IRON/SULFUR CLUSTER

ChemComp-AMP:
ADENOSINE MONOPHOSPHATE / AMP*YM

ChemComp-MG:
Unknown entry

PDB-1cq3:
STRUCTURE OF A SOLUBLE SECRETED CHEMOKINE INHIBITOR, VCCI, FROM COWPOX VIRUS

PDB-1cq4:
CI2 MUTANT WITH TETRAGLUTAMINE (MGQQQQGM) REPLACING MET59

Source
  • homo sapiens (human)
KeywordsBIOSYNTHETIC PROTEIN / Complex / Purine synthesis / phosphoribosyltransferase

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