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TitleCryo-EM structure of the native assembled Mfa type V pilus from the periodontal pathogen Porphyromonas gingivalis.
Journal, issue, pagesCommun Biol, Year 2026
Publish dateDec 8, 2025
AuthorsSatoshi Shibata / Hideyuki Matsunami / Kazuhisa Ouhara / Yuri Taniguchi / Makoto Tokoro Schreiber / Alejandro Villar-Brillones / Koji Nakayama / Mikio Shoji / Matthias Wolf /
PubMed AbstractPorphyromonas gingivalis is a primary pathogen causing periodontal disease. The cell has two kinds of type V pili, the Fim pilus and the Mfa pilus, both of which play essential roles in colonization, ...Porphyromonas gingivalis is a primary pathogen causing periodontal disease. The cell has two kinds of type V pili, the Fim pilus and the Mfa pilus, both of which play essential roles in colonization, biofilm formation, and pathogenicity. The functional polymerized structure of the Fim pilus is known, whereas the structure and assembly mechanism of the Mfa pilus remain unclear. Here, we show the structure of the polymerized recombinant Mfa1 stalk pilin determined by cryo-electron microscopy at 3.0 Å resolution. The atomic model of the Mfa1 filament reveals that Mfa1 pilins polymerize by protease-mediated strand exchange and retain a Ca ion in the metal-binding pocket, which modulates immune recognition of the Mfa pilus by human cells. Furthermore, we elucidated the three-dimensional architecture of the streptococcal-binding region on the Mfa pilus. Our results further strengthen evidence that protease-mediated strand exchange is the universal assembly mechanism of type V pili. Our structure of the polymerized Mfa pilus, which represents the functional state on the cell surface, provides targets for antimicrobial drug design to treat periodontal disease and P. gingivalis-related systemic diseases.
External linksCommun Biol / PubMed:42336997
MethodsEM (single particle)
Resolution2.99 Å
Structure data

67574
EMDB Unreleased entry

21co

EMDB-67574, PDB-21co:
Mfa1 type V pilus from P.gingivalis
Method: EM (single particle) / Resolution: 2.99 Å

Chemicals

ChemComp-CA:
Unknown entry

Source
  • porphyromonas gingivalis atcc 33277 (bacteria)
KeywordsCELL ADHESION / Type V pilus / Mfa1 / Porphyromonas gingivalis / ATCC33277

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