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TitleMolecular mechanisms of transhydrogenase activity and allosteric regulation in eukaryotic type II PHGDH Ser33.
Journal, issue, pagesNat Commun, Year 2026
Publish dateJun 22, 2023 (structure data deposition date)
AuthorsPerrone, S. / Cifuente, J.O. / Mastrella, L. / Marina, A. / Trastoy, B. / Becker-Kettern, J. / Conrotte, J.F. / Alcaide-Jimenez, A. / Corzana, F. / Glaab, E. ...Perrone, S. / Cifuente, J.O. / Mastrella, L. / Marina, A. / Trastoy, B. / Becker-Kettern, J. / Conrotte, J.F. / Alcaide-Jimenez, A. / Corzana, F. / Glaab, E. / Guerin, M.E. / Linster, C.L.
External linksNat Commun / PubMed:42321184
MethodsX-ray diffraction
Resolution2.51 - 3.19 Å
Structure data

PDB-8pin:
Crystal structure of Ser33
Method: X-RAY DIFFRACTION / Resolution: 3.19 Å

PDB-8pio:
Crystal structure of Ser33 in complex with PHP (3-phosphohydroxypyruvate)
Method: X-RAY DIFFRACTION / Resolution: 2.6 Å

PDB-8pir:
Crystal structure of Ser33 in complex with 3-PGA (3-phosphoglycerate)
Method: X-RAY DIFFRACTION / Resolution: 2.7 Å

PDB-8pis:
Crystal structure of Ser33 in complex with L-Serine
Method: X-RAY DIFFRACTION / Resolution: 2.69 Å

PDB-8q2i:
Crystal structure of Ser33 in complex 2HG (2-hydroxyglutarate) and Serine
Method: X-RAY DIFFRACTION / Resolution: 2.51 Å

Chemicals

ChemComp-NAD:
NICOTINAMIDE-ADENINE-DINUCLEOTIDE / NAD*YM

ChemComp-PI:
HYDROGENPHOSPHATE ION

ChemComp-HPV:
2-oxo-3-(phosphonooxy)propanoic acid

ChemComp-GOL:
GLYCEROL

ChemComp-HOH:
WATER

ChemComp-3PG:
3-PHOSPHOGLYCERIC ACID

ChemComp-BU1:
1,4-BUTANEDIOL

ChemComp-SER:
SERINE

ChemComp-2HG:
(2R)-2-hydroxypentanedioic acid

Source
  • saccharomyces cerevisiae (brewer's yeast)
KeywordsCYTOSOLIC PROTEIN / Yeast protein / Enzyme Protein

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