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Structure paper

TitleDefinition of the catalytic cleft and product profile of a GH5_5 cellulase from Thermoascus aurantiacus.
Journal, issue, pagesActa Crystallogr D Struct Biol, Year 2026
Publish dateFeb 4, 2022 (structure data deposition date)
AuthorsCollet, L. / Denys, A. / Oudjama, Y. / Sifennasr, K. / Vander Wauven, C. / Dutoit, R.
External linksActa Crystallogr D Struct Biol / PubMed:42101894
MethodsX-ray diffraction
Resolution1.18 - 1.61 Å
Structure data

PDB-7r28:
Crystal structure of Ta_Cel5A E133Q Y200F variant, apoform
Method: X-RAY DIFFRACTION / Resolution: 1.22 Å

PDB-7r29:
Crystal structure of TaCel5A E133Q Y200F variant with covalently linked cellotriose
Method: X-RAY DIFFRACTION / Resolution: 1.28 Å

PDB-7r2a:
Crystal structure of Ta_Cel5A Y200F variant, apoform
Method: X-RAY DIFFRACTION / Resolution: 1.3 Å

PDB-7r2c:
Crystal structure of TaCel5A Y200F variant in complex with 2-chloro-4-nitrophenyl-glucose
Method: X-RAY DIFFRACTION / Resolution: 1.48 Å

PDB-7r2d:
Crystal structure of TaCel5A E133A variant in complex with cellopentaose
Method: X-RAY DIFFRACTION / Resolution: 1.61 Å

PDB-9f0n:
Crystal structure of Ta_Cel5A E133A variant, apoform
Method: X-RAY DIFFRACTION / Resolution: 1.18 Å

Chemicals

ChemComp-SO4:
SULFATE ION

ChemComp-HOH:
WATER

ChemComp-GOL:
GLYCEROL

ChemComp-TRS:
2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL / pH buffer*YM

ChemComp-P9P:
2-chloranyl-4-nitro-phenol

ChemComp-BGC:
beta-D-glucopyranose

Source
  • thermoascus aurantiacus atcc 26904 (fungus)
KeywordsHYDROLASE / cellulase / GH5_5 family / GH-A clan / (a/b)8 fold / Thermoascus aurantiacus / glycoside hydrolase / (a/b)8 barrel / (b/a)8 barrel / TIM barrel

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