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TitleStructural basis for DNA processing and membrane translocation by ComEC in natural transformation.
Journal, issue, pagesScience, Vol. 392, Issue 6795, Page 311-316, Year 2026
Publish dateApr 16, 2026
AuthorsHisato Hirano / Naoko Tsuji / Shinobu Chiba / Osamu Nureki /
PubMed AbstractNatural transformation is one of the major pathways of horizontal gene transfer in bacteria, enabling the acquisition of extracellular DNA and its integration into the host genome. ComEC is a ...Natural transformation is one of the major pathways of horizontal gene transfer in bacteria, enabling the acquisition of extracellular DNA and its integration into the host genome. ComEC is a membrane protein responsible for DNA translocation in this process, yet its precise function and structure have remained elusive. Here, we report cryo-electron microscopy structures of ComEC in DNA-free, single-stranded DNA (ssDNA)-bound, and double-stranded DNA (dsDNA)-bound forms, together with biochemical analyses. These structures reveal that ComEC cleaves one strand of dsDNA at its extracellular domain and guides the remaining strand into a positively charged pore formed within the membrane domain. These findings provide a structural basis for the long-hypothesized roles of ComEC in both DNA processing and translocation across the inner membrane during natural transformation.
External linksScience / PubMed:41990170
MethodsEM (single particle)
Resolution2.71 - 3.23 Å
Structure data

64944

9vc6

EMDB-64944, PDB-9vc6:
Cryo-EM structure of Bacillus halodurans ComEC
Method: EM (single particle) / Resolution: 2.71 Å

64949

9vc9

EMDB-64949, PDB-9vc9:
Cryo-EM structure of Shewanella decolorationis ComEC
Method: EM (single particle) / Resolution: 2.77 Å

64951

9vcb

EMDB-64951, PDB-9vcb:
Cryo-EM structure of Shewanella putrefaciens ComEC in complex with ssDNA
Method: EM (single particle) / Resolution: 2.82 Å

64953

9vcf

EMDB-64953, PDB-9vcf:
Cryo-EM structure of Shewanella putrefaciens ComEC in complex with dsDNA
Method: EM (single particle) / Resolution: 3.23 Å

Chemicals

ChemComp-AV0:
Lauryl Maltose Neopentyl Glycol

ChemComp-ZN:
Unknown entry

Source
  • halalkalibacterium halodurans c-125 (bacteria)
  • shewanella decolorationis (bacteria)
  • shewanella putrefaciens (bacteria)
  • synthetic construct (others)
KeywordsMEMBRANE PROTEIN / Competence / DNA channel / Hydrolase

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