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TitleModulation of succinyl-CoA:3-ketoacid CoA transferase activity by a single amino acid residue in acetate:succinate CoA transferase from Trypanosoma brucei, the causative agent of African sleeping sickness.
Journal, issue, pagesProtein Sci., Vol. 35, Page e70463-e70463, Year 2026
Publish dateJan 18, 2023 (structure data deposition date)
AuthorsMochizuki, K. / Inaoka, D.K. / Fukuda, K. / Kurasawa, H. / Iyoda, K. / Nakai, U. / Harada, S. / Balogun, E.O. / Bringaud, F. / Boshart, M. ...Mochizuki, K. / Inaoka, D.K. / Fukuda, K. / Kurasawa, H. / Iyoda, K. / Nakai, U. / Harada, S. / Balogun, E.O. / Bringaud, F. / Boshart, M. / Sakura, T. / Hirayama, K. / Kita, K. / Shiba, T.
External linksProtein Sci. / PubMed:41556494
MethodsX-ray diffraction
Resolution2.4 - 2.79 Å
Structure data

PDB-8i3y:
Crystal structure of ASCT from Trypanosoma brucei in complex with Succinyl-CoA.
Method: X-RAY DIFFRACTION / Resolution: 2.4 Å

PDB-8i40:
Crystal structure of ASCT from Trypanosoma brucei in complex with CoA.
Method: X-RAY DIFFRACTION / Resolution: 2.79 Å

PDB-9xmy:
Crystal structure of ASCT D62N mutant from Trypanosoma brucei in complex with succinyl-CoA and acetylacetone.
Method: X-RAY DIFFRACTION / Resolution: 2.6 Å

Chemicals

ChemComp-SCA:
SUCCINYL-COENZYME A

ChemComp-CA:
Unknown entry

ChemComp-SIN:
SUCCINIC ACID

ChemComp-HOH:
WATER

ChemComp-COA:
COENZYME A

ChemComp-ACT:
ACETATE ION

ChemComp-AAE:
ACETOACETIC ACID

Source
  • trypanosoma brucei brucei treu927 (eukaryote)
  • trypanosoma brucei brucei (strain 927/4 gutat10.1) (eukaryote)
KeywordsELECTRON TRANSPORT / Trypanosoma brucei / Acetate:succinate CoA transferase

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