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TitleStructural basis for the assembly and energy transfer between the cyanobacterial PSI core and the double-layered IsiA proteins.
Journal, issue, pagesNat Commun, Vol. 17, Issue 1, Page 592, Year 2025
Publish dateDec 20, 2025
AuthorsLong Si / Yingyue Zhang / Xiaodong Su / Xuelin Zhao / Xiaomin An / Lu-Ning Liu / Peng Cao / Mei Li /
PubMed AbstractIron-limitation is a common stress factor in natural environments. To survive under iron-starved conditions, cyanobacteria overexpress iron stress-induced protein A (IsiA), which is crucial for light- ...Iron-limitation is a common stress factor in natural environments. To survive under iron-starved conditions, cyanobacteria overexpress iron stress-induced protein A (IsiA), which is crucial for light-harvesting and photoprotection. Multiple IsiA proteins form a single- or double-layered architecture encircling the photosystem I (PSI) core, forming various PSI-IsiA supercomplexes. The assembly and energy transfer mechanisms of double-layered PSI-IsiA supercomplexes remain unelucidated. Here, we present high-resolution structures of two PSI-IsiA supercomplexes isolated from the cyanobacterium Thermosynechococcus elongatus BP-1 cultured under iron-starved conditions. The PSI-IsiA complex contains a trimeric PSI core surrounded by 43 IsiA subunits assembled into a closed double-ring. The PSI-IsiA complex contains 13 IsiA proteins arranged in a double-layered architecture attached to the monomeric PSI core. Atomic force microscopy demonstrates the presence and distribution of different PSI-IsiA complexes within native thylakoid membranes isolated from iron-starved cells. Our findings provide insights into the structural variability and adaptive mechanisms of PSI-IsiA complexes.
External linksNat Commun / PubMed:41422266 / PubMed Central
MethodsEM (single particle)
Resolution3.4 - 3.5 Å
Structure data

63527

9lzj

EMDB-63527, PDB-9lzj:
The PSI3-IsiA43 complex with a closed double ring of IsiA proteins bound to a trimeric PSI core
Method: EM (single particle) / Resolution: 3.4 Å

63528

9lzk

EMDB-63528, PDB-9lzk:
The PSI1-IsiA13 complex with double-layered IsiA proteins bound to the monomeric PSI core
Method: EM (single particle) / Resolution: 3.5 Å

Chemicals

ChemComp-CLA:
CHLOROPHYLL A

ChemComp-PQN:
PHYLLOQUINONE

ChemComp-SF4:
IRON/SULFUR CLUSTER

ChemComp-BCR:
BETA-CAROTENE

ChemComp-LHG:
1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE / phospholipid*YM

ChemComp-LMU:
DODECYL-ALPHA-D-MALTOSIDE / detergent*YM

ChemComp-SQD:
1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL

ChemComp-LMG:
1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE

ChemComp-CA:
Unknown entry

Source
  • thermosynechococcus vestitus bp-1 (bacteria)
KeywordsPHOTOSYNTHESIS / Photosystem-antenna Complex

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