EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structural and catalytic diversity of coronavirus proofreading exoribonuclease.
ジャーナル・号・ページ	Nat Commun, Vol. 17, Issue 1, Page 452, Year 2025
掲載日	2025年12月8日
著者	Yu Li / Xiaocong Cao / Lauren M Recker / Yang Yang / Chang Liu /
PubMed 要旨	The coronavirus proofreading exoribonuclease (ExoN) is essential for genome fidelity and immune evasion of the viruses. Despite its critical roles in the viral life cycle, it is unclear how ExoNs ...The coronavirus proofreading exoribonuclease (ExoN) is essential for genome fidelity and immune evasion of the viruses. Despite its critical roles in the viral life cycle, it is unclear how ExoNs across different coronaviruses diverge in their structures and catalytic properties, which may lead to differences in viral genome mutation rates and, consequently, viral fitness, immune evasion, and resistance to antiviral drugs. Here, we present comparative structural and biochemical analyses of ExoNs between two most representative human coronaviruses, Middle East Respiratory Syndrome Coronavirus (MERS-CoV) from the merbecovirus subgenus and SARS-CoV-2 from the sarbecovirus subgenus. Our results reveal a markedly lower catalytic activity of ExoN from MERS-CoV than that from SARS-CoV-2. The molecular basis of such a divergence across the two coronaviruses is unveiled by the cryo-EM structures of MERS-CoV ExoN in complex with RNA substrates bearing different 3'-end base pairs or mismatch, which represent the first set of ExoN structures from a coronavirus outside the sarbecovirus subgenus. Our findings also identify two highly conserved structural determinants that dictate efficient excision of different nucleotides at the 3' terminus of RNA substrates by coronavirus ExoNs, a property that is pivotal for their roles in both viral RNA proofreading and immune evasion.
リンク	Nat Commun / PubMed:41361186 / PubMed Central
手法	EM (単粒子)
解像度	2.5 - 2.94 Å
構造データ	72775 9yck EMDB-72775, PDB-9yck: Cryo-EM structure of MERS-CoV nsp10-nsp14 (E191A) in complex with T20P15 RNA, monomeric form 手法: EM (単粒子) / 解像度: 2.8 Å 72776 9ycl EMDB-72776, PDB-9ycl: Cryo-EM structure of MERS-CoV nsp10-nsp14 (E191A) in complex with T20P15 RNA, dimeric form 手法: EM (単粒子) / 解像度: 2.5 Å 72777 9ycm EMDB-72777, PDB-9ycm: Cryo-EM structure of MERS-CoV nsp10-nsp14 (E191A) in complex with T20P14-U RNA 手法: EM (単粒子) / 解像度: 2.94 Å 72778 9ycn EMDB-72778, PDB-9ycn: Cryo-EM structure of MERS-CoV nsp10-nsp14 (E191A) in complex with T20P14-A RNA 手法: EM (単粒子) / 解像度: 2.88 Å 72779 9yco EMDB-72779, PDB-9yco: Cryo-EM structure of MERS-CoV nsp10-nsp14 (E191A) in complex with T20P14-G RNA 手法: EM (単粒子) / 解像度: 2.88 Å
化合物	ChemComp-ZN: Unknown entry ChemComp-MG: Unknown entry
由来	middle east respiratory syndrome-related coronavirus (ウイルス)
キーワード	Viral Protein/RNA / MERS-CoV / exoribonuclease / proofreading / coronavirus / RNA / VIRAL PROTEIN / Viral Protein-RNA complex / RNA/VIRAL PROTEIN / RNA-VIRAL PROTEIN complex