EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	PCNA is a nucleotide exchange factor for the clamp loader ATPase complex.
ジャーナル・号・ページ	Proc Natl Acad Sci U S A, Vol. 122, Issue 46, Page e2518834122, Year 2025
掲載日	2025年11月18日
著者	Joshua Pajak / Jacob T Landeck / Xingchen Liu / Krishna Anand / Sasha Litvak / Brian A Kelch /
PubMed 要旨	All life requires loading ring-shaped sliding clamp protein complexes onto DNA. The sliding clamp loader is a conserved AAA+ ATPase that binds the sliding clamp, opens the ring, and places it onto ...All life requires loading ring-shaped sliding clamp protein complexes onto DNA. The sliding clamp loader is a conserved AAA+ ATPase that binds the sliding clamp, opens the ring, and places it onto DNA. While recent structural work on both the canonical and "alternative" clamp loaders has shed light into how these machines perform their task once, it remains unclear how clamp loaders are recycled to load multiple sliding clamps. Here, we present structures of the clamp loader Replication Factor C (RFC) in absence of sliding clamp or supplemented nucleotide. Our structures indicate that RFC holds onto ADP tightly in at least two of its four ATPase active sites, suggesting that nucleotide exchange is regulated. Our molecular dynamics simulations and biochemical data indicate that binding of the sliding clamp Proliferating Cell Nuclear Antigen (PCNA) causes rapid exchange of tightly bound ADP. Our data suggest that PCNA acts as a nucleotide exchange factor (NEF) by prying apart adjacent subunits, providing a pathway for ADP release. We propose that, by using its own substrate as a NEF, RFC excludes off-pathway states that would arise from binding DNA prior to PCNA.
リンク	Proc Natl Acad Sci U S A / PubMed:41231947 / PubMed Central
手法	EM (単粒子)
解像度	2.57 - 2.63 Å
構造データ	71572 9peo EMDB-71572, PDB-9peo: Structure of the S. cerevisiae clamp loader Replication Factor C (RFC) with mixed nucleotide occupancy 手法: EM (単粒子) / 解像度: 2.57 Å 71574 9per EMDB-71574, PDB-9per: Structure of the S. cerevisiae clamp loader Replication Factor C (RFC) with mixed nucleotide occupancy 手法: EM (単粒子) / 解像度: 2.57 Å 71575 9pes EMDB-71575, PDB-9pes: Structure of the S. cerevisiae clamp loader Replication Factor C (RFC) with mixed nucleotide occupancy 手法: EM (単粒子) / 解像度: 2.59 Å 71576 9pet EMDB-71576, PDB-9pet: Structure of the S. cerevisiae clamp loader Replication Factor C (RFC) with mixed nucleotide occupancy 手法: EM (単粒子) / 解像度: 2.57 Å 71577 9peu EMDB-71577, PDB-9peu: Structure of the S. cerevisiae clamp loader Replication Factor C (RFC) with mixed nucleotide occupancy 手法: EM (単粒子) / 解像度: 2.62 Å 71578 9pev EMDB-71578, PDB-9pev: Structure of the S. cerevisiae clamp loader Replication Factor C (RFC) with mixed nucleotide occupancy 手法: EM (単粒子) / 解像度: 2.63 Å
化合物	ChemComp-ACT: ACETATE ION ChemComp-ADP: ADENOSINE-5'-DIPHOSPHATE / ADP, エネルギー貯蔵分子YM ChemComp-GDP: GUANOSINE-5'-DIPHOSPHATE / GDP, エネルギー貯蔵分子YM
由来	saccharomyces cerevisiae (パン酵母)
キーワード	REPLICATION / AAA+ ATPase / Rossmann Fold / Complex / DNA replication