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Structure paper

TitleStructure and encapsulation of carbonic anhydrase within the α-carboxysome.
Journal, issue, pagesProc Natl Acad Sci U S A, Vol. 122, Issue 46, Page e2523723122, Year 2025
Publish dateNov 18, 2025
AuthorsPei Cing Ng / Oluwatobi Adegbite / Tianpei Li / Arnaud Baslé / Jon Marles-Wright / Lu-Ning Liu /
PubMed AbstractCarboxysomes in cyanobacteria and certain proteobacteria enable efficient CO fixation by encapsulating ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) and carbonic anhydrase (CA) within a ...Carboxysomes in cyanobacteria and certain proteobacteria enable efficient CO fixation by encapsulating ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) and carbonic anhydrase (CA) within a semipermeable shell. Sequestered CA catalyze the rapid interconversion of CO and HCO, supplying elevated levels of CO to boost Rubisco carboxylation. Despite its essential role, the structure and encapsulation of CA within carboxysomes remain poorly understood. Here, we determined the molecular structure of α-carboxysomal CA from the model chemoautotrophic bacterium (CsoSCA). CsoSCA adopts a trimer-of-dimers oligomeric structure without the incorporation of a zinc ion at its symmetric center. Using synthetic minishells, we demonstrate that CsoSCA interacts with the CsoS1A shell hexamer and is incorporated into the minishells at the inner surface, independent of the CsoS2 linker protein. CsoSCA truncations suggest nonspecific interactions between CsoSCA and CsoS1A. We further show that CsoSCA bridges Rubisco and the shell facets. Our study offers insights into the assembly and encapsulation mechanisms of α-carboxysomes and provides the framework for reprogramming carboxysome structures for synthetic biology and biotechnological applications.
External linksProc Natl Acad Sci U S A / PubMed:41223214 / PubMed Central
MethodsEM (single particle)
Resolution1.82 - 3.1 Å
Structure data

51067

9g4t

EMDB-51067, PDB-9g4t:
Beta carbonic anhydrase CsoSCA from the Halothiobacillus neapolitanus alpha-carboxysome
Method: EM (single particle) / Resolution: 2.51 Å

51633

9gvc

EMDB-51633, PDB-9gvc:
Cryo-EM structure of Halothiobacillus neapolitanus alpha-carboxysome T=4 mini-shell containing CTD truncated mutant of CsoSCA
Method: EM (single particle) / Resolution: 1.82 Å

51641

9gw1

EMDB-51641, PDB-9gw1:
Cryo-EM structure of alpha-carboxysome T=4 mini-shell containing CTD only mutant of CsoSCA
Method: EM (single particle) / Resolution: 3.1 Å

Chemicals

ChemComp-ZN:
Unknown entry

ChemComp-HOH:
WATER

Source
  • halothiobacillus neapolitanus (bacteria)
  • streptococcus sp. 'group g' (bacteria)
KeywordsLYASE / Carbonic anhydrase / Carboxysome / rubisco / STRUCTURAL PROTEIN

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