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TitleFilamentous structure of the CotVW complex, the crust proteins of the Bacillus subtilis endospore.
Journal, issue, pagesJ Biol Chem, Vol. 301, Issue 11, Page 110714, Year 2025
Publish dateSep 11, 2025
AuthorsEunbyul Jo / Doyeon Kim / Yeongjin Baek / Migak Park / Hyojeong Lee / Nam-Chul Ha /
PubMed AbstractThe endospores of Bacillus subtilis are encased in a multilayered protective structure comprising core, cortex, inner and outer coats, and an outermost crust. Among the proteins required for crust ...The endospores of Bacillus subtilis are encased in a multilayered protective structure comprising core, cortex, inner and outer coats, and an outermost crust. Among the proteins required for crust formation, CotV and CotW are unique to B. subtilis and are hypothesized to be instrumental in maintaining spore surface integrity. However, their structural organization and functional mechanisms remain unclear. This study determined the cryo-EM structure of the CotVW complex and revealed its filamentous helical architecture. Structural analysis showed that CotVW possesses a negatively charged surface that enables pH-dependent binding interactions. Specifically, at pH 6.0, CotVW engages in electrostatic interactions with histidine and positively charged residues, suggesting a potential regulatory mechanism influenced by the environmental pH. Our results elucidate the molecular basis of CotVW function in B. subtilis spore crust formation, highlighting its role in spore surface organization. This study advances our understanding of the spore coat architecture and may inform future research on bacterial spore resilience and structural adaptation.
External linksJ Biol Chem / PubMed:40945727 / PubMed Central
MethodsEM (helical sym.)
Resolution3.32 Å
Structure data

63065

9lgh

EMDB-63065, PDB-9lgh:
Cryo-EM structure of CotVW filament, bacillus subtilis endospore protein
Method: EM (helical sym.) / Resolution: 3.32 Å

Source
  • bacillus subtilis subsp. subtilis str. 168 (bacteria)
KeywordsSTRUCTURAL PROTEIN / bacillus / spore protein / complex / filament / helical / PROTEIN FIBRIL

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