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-Structure paper
| タイトル | Unusual cell surfaces, pili, and archaella of Thermoplasmatales archaea. |
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| ジャーナル・号・ページ | ISME J, Vol. 19, Issue 1, Year 2025 |
| 掲載日 | 2025年1月2日 |
著者 | Matthew C Gaines / Michail N Isupov / Mathew McLaren / Risat Ul Haque / Alejandra Recalde / Rafael Bargiela / Vicki A M Gold / Sonja-Verena Albers / Peter N Golyshin / Olga V Golyshina / Bertram Daum / ![]() |
| PubMed 要旨 | Archaea of the order Thermoplasmatales push the boundaries of our current knowledge of prokaryotic life. They show distinct cellular plasticity, heterogenous cell morphologies, and lack a ...Archaea of the order Thermoplasmatales push the boundaries of our current knowledge of prokaryotic life. They show distinct cellular plasticity, heterogenous cell morphologies, and lack a paracrystalline S-layer. As the S-layer has previously been implicated in acting as a stator scaffold for filaments driving cellular propulsion, particularly archaella, we asked whether the absence of an S-layer precludes the formation of functional archaella or pili in Thermoplasmatales. Using cryoEM, we investigated the two Thermoplasmatales species Cuniculiplasma divulgatum and Oxyplasma meridianum. We found that these species indeed generate pili and archaella and that the latter likely function in cellular propulsion. Whereas C. divulgatum produces pili with terminal hooks using a unique assembly machinery, O. meridianum generates unusually wide, "barbed" archaella with a high degree of glycosylation. Our results show that for the generation of functional archaella and pili, a canonical S-layer is not necessary. |
リンク | ISME J / PubMed:40801261 / PubMed Central |
| 手法 | EM (らせん対称) |
| 解像度 | 2.54 - 2.61 Å |
| 構造データ | EMDB-19112, PDB-8rey: EMDB-19168, PDB-8rh5: |
| 化合物 | ![]() ChemComp-GAL: |
| 由来 |
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キーワード | PROTEIN FIBRIL / cell surface appendage / N-glycosylation |
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cuniculiplasma divulgatum (古細菌)
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