EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Cryo-EM captures early intermediate steps in dynein activation by LIS1.
ジャーナル・号・ページ	Nat Commun, Vol. 16, Issue 1, Page 7054, Year 2025
掲載日	2025年8月1日
著者	Kendrick H V Nguyen / Eva P Karasmanis / Agnieszka A Kendrick / Samara L Reck-Peterson / Andres E Leschziner /
PubMed 要旨	Cytoplasmic dynein-1 (dynein) is an essential molecular motor in eukaryotic cells. Dynein primarily exists in an autoinhibited Phi state and requires conformational changes to assemble with its ...Cytoplasmic dynein-1 (dynein) is an essential molecular motor in eukaryotic cells. Dynein primarily exists in an autoinhibited Phi state and requires conformational changes to assemble with its cofactors and form active transport complexes. LIS1, a key dynein regulator, enhances dynein activation and assembly. Using cryo-EM and a human dynein-LIS1 sample incubated with ATP, we map the conformational landscape of dynein activation by LIS1 and identify an early intermediate state that we propose precedes the previously identified dynein-LIS1 Chi state. Mutations that disrupt this species, which we termed "Pre-Chi", lead to motility defects in vitro, emphasizing its functional importance. Together, our findings provide insights into how LIS1 relieves dynein autoinhibition during the activation pathway.
リンク	Nat Commun / PubMed:40750582 / PubMed Central
手法	EM (単粒子)
解像度	2.7 - 6.2 Å
構造データ	47342 9dzy EMDB-47342, PDB-9dzy: Cryo-EM structure of Pre-Chi dynein bound to Lis1 手法: EM (単粒子) / 解像度: 3.1 Å 47360 9e0k EMDB-47360, PDB-9e0k: Cryo-EM structure of human cytoplasmic dynein-1 in the presence of ATP 手法: EM (単粒子) / 解像度: 3.2 Å 47370 9e0t EMDB-47370, PDB-9e0t: Cryo-EM structure of human cytoplasmic dynein-1 bound to LIS1 in the presence of ATP 手法: EM (単粒子) / 解像度: 3.1 Å 47371 9e0u EMDB-47371, PDB-9e0u: Cryo-EM structure of human cytoplasmic dynein-1 in the presence of ATP 手法: EM (単粒子) / 解像度: 3.4 Å 47372 9e0w EMDB-47372, PDB-9e0w: Cryo-EM structure of human cytoplasmic dynein-1 bound to LIS1 in the presence of ATP 手法: EM (単粒子) / 解像度: 3.2 Å 47373 9e0x EMDB-47373, PDB-9e0x: Cryo-EM structure of Phi dynein 手法: EM (単粒子) / 解像度: 2.7 Å 47377 9e0y EMDB-47377, PDB-9e0y: Cryo-EM structure of human cytoplasmic dynein-1 in the presence of ATP 手法: EM (単粒子) / 解像度: 3.4 Å 47429 9e22 EMDB-47429, PDB-9e22: Cryo-EM structure of human cytoplasmic dynein-1 bound to LIS1 in the presence of ATP 手法: EM (単粒子) / 解像度: 3.3 Å 47430 9e23 EMDB-47430, PDB-9e23: Cryo-EM structure of Pre-Chi dynein tail 手法: EM (単粒子) / 解像度: 6.2 Å 47443 9e28 EMDB-47443, PDB-9e28: Cryo-EM structure of Phi dynein tail 手法: EM (単粒子) / 解像度: 4.4 Å
化合物	ChemComp-ADP: ADENOSINE-5'-DIPHOSPHATE / ADP / ADP, エネルギー貯蔵分子YM ChemComp-ATP: ADENOSINE-5'-TRIPHOSPHATE / ATP / ATP, エネルギー貯蔵分子YM ChemComp-MG: Unknown entry / マグネシウムジカチオン
由来	homo sapiens (ヒト)
キーワード	MOTOR PROTEIN / Complex / Human