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-Structure paper
| タイトル | In situ cryo-electron tomography reveals the progressive biogenesis of basal bodies and cilia in mouse ependymal cells. |
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| ジャーナル・号・ページ | Nat Commun, Vol. 16, Issue 1, Page 5932, Year 2025 |
| 掲載日 | 2025年7月1日 |
 著者 | Shanshan Ma / Luan Li / Zhixun Li / Shenjia Luo / Qi Liu / Wenjing Du / Benhua Qiu / Miao Gui / Xueliang Zhu / Qiang Guo /  |
| PubMed 要旨 | Cilia, essential organelles for cell motility and signaling, comprise an axoneme extended from the basal body (BB). The assembly process of BBs and axonemes during ciliogenesis, however, remains ...Cilia, essential organelles for cell motility and signaling, comprise an axoneme extended from the basal body (BB). The assembly process of BBs and axonemes during ciliogenesis, however, remains largely unknown due to the lack of structural information. Here, we leverage in-situ cryo-electron tomography to capture within mouse ependymal cells the dynamic processes of BB biogenesis and multiciliogenesis at various stages. This approach enables 3D visualization of the complete motile machinery, revealing the continuous microtubule-based scaffold from BBs to axonemes at sub-nanometer resolution with unprecedented structural details. Furthermore, we elucidate along BBs and cilia heterogeneous landscapes of microtubule-binding proteins underlying the establishment of structural periodicity and diverse subregions. Notably, the chronological binding patterns of microtubule-inner proteins (e.g., CEP41) correlate with the progressive assembly of ciliary beating machinery. We also resolve a substructure that borders the BB and the axoneme. Our findings provide key insights into intricate orchestrations during ciliogenesis. |
 リンク | Nat Commun / PubMed:40593728 / PubMed Central |
| 手法 | EM (サブトモグラム平均) / EM (トモグラフィー) |
| 解像度 | 7.6 - 14.2 Å |
| 構造データ |  EMDB-39652: Subtomogram averaging of 8-nm-repeat triplet microtubule with only A and B tubules within basal bodies from mouse ependymal cells  EMDB-39653: Subtomogram averaging of 8-nm-repeat triplet microtubule with inner scaffold and complete C tubule within basal bodies from mouse ependymal cells  EMDB-39654: Subtomogram averaging of 8-nm-repeat triplet microtubule with pinhead within basal bodies from mouse ependymal cells  EMDB-39655: Subtomogram averaging of 8-nm-repeat triplet microtubule with inner scaffold and incomplete C tubule within basal bodies from mouse ependymal cells  EMDB-39656: Subtomogram averaging of 8-nm-repeat doublet microtubule within the transition zone from mouse ependymal cells  EMDB-39657: Subtomogram averaging of 48-nm-repeat doublet microtubule from mouse ependymal cells  EMDB-39658: Subtomogram averaging of Cep41 bound to microtubules from mouse ependymal cells  EMDB-39659: A tomogram of ciliogenesis in the cytoplasm of mouse ependymal cells  EMDB-39660: A tomogram of deuterosome-dependent basal body biogenesis in mouse ependymal cells  EMDB-63282: A tomogram of basal body docked into plasma membrane and templating a cilium in mouse ependymal cells  EMDB-63283: A tomogram of basal bodies docked into plasma membrane in mouse ependymal cells  EMDB-63284: A tomogram of deuterosome-dependent basal body biogenesis in mouse ependymal cells  EMDB-63285: A tomogram of deuterosome-dependent basal body biogenesis in mouse ependymal cells  EMDB-63286: A tomogram of deuterosome-dependent basal body biogenesis in mouse ependymal cells  EMDB-63287: A tomogram of basal bodies docked into plasma membrane and templating cilia in mouse ependymal cells  EMDB-63288: A tomogram of deuterosome-dependent basal body biogenesis in mouse ependymal cells  EMDB-63289: A tomogram of deuterosome-dependent basal body biogenesis in mouse ependymal cells |
| 由来 |
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Mus musculus (ハツカネズミ)