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TitleNatural Design of a Stabilized Cross-β Fold: Structure of the FuA FapC from Pseudomonas Sp. UK4 Reveals a Critical Role for Stacking of Imperfect Repeats.
Journal, issue, pagesAdv Mater, Vol. 37, Issue 34, Page e2505503, Year 2025
Publish dateJun 11, 2025
AuthorsYanting Jiang / Samuel Peña-Díaz / Zhefei Zhang / Anders Ogechi Hostrup Daugberg / Marcos López Hernández / Janni Nielsen / Qiaojie Huang / Shenghan Qin / Morten K D Dueholm / Mingdong Dong / Jan Skov Pedersen / Qin Cao / Daniel E Otzen / Huabing Wang /
PubMed AbstractAn essential structural component of bacterial biofilms is functional amyloid (FuA), which also has great potential as an engineerable nano-biomaterial. However, experimentally based high resolution ...An essential structural component of bacterial biofilms is functional amyloid (FuA), which also has great potential as an engineerable nano-biomaterial. However, experimentally based high resolution structures of FuA that resolve individual residues are lacking. A fully experimentally based 3.2 Å resolution cryo-electron microscopy density map of the FuA protein FapC from Pseudomonas sp. UK4 is presented, which reveals a Greek key-shaped protofilament. The structure supports bioinformatic identification of conserved motifs and is broadly consistent with the AlphaFold prediction but with important modifications. Each FapC monomer consists of three imperfect repeats (IRs), with each repeat forming one cross-β layer. An array of highly conserved Asn and Gln residues with an extensive H-bonding network underpins this conserved Greek key-shape and reveals the role of heterogeneous cross-β stacking in amyloid cross-seeding. The covariation of residues in the hydrophobic core among different IRs suggests a cooperative monomer folding process during fibril elongation, while heterogeneous stacking of IRs reduces charge repulsion between layers to stabilize the monomer fold. The FapC fibrils show intrinsic catalytic activity and strain-dependent nanomechanical properties. Combined with mutagenesis data, the structure provides mechanistic insights into formation of FapC FuA from disordered monomers and a structural foundation for the design of novel biomaterials.
External linksAdv Mater / PubMed:40495649 / PubMed Central
MethodsEM (helical sym.)
Resolution3.2 Å
Structure data

63855

9u4u

EMDB-63855, PDB-9u4u:
Structure of the functional amyloid FapC from Pseudomonas sp.UK4
Method: EM (helical sym.) / Resolution: 3.2 Å

Source
  • pseudomonas sp. uk4 (bacteria)
KeywordsPROTEIN FIBRIL / Functional amyloid

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