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Structure paper

TitleMechanism of DNA degradation by CBASS Cap5 endonuclease immune effector.
Journal, issue, pagesNat Commun, Vol. 16, Page 5243-5243, Year 2025
Publish dateSep 5, 2024 (structure data deposition date)
AuthorsRechkoblit, O. / Sciaky, D. / Ni, M. / Li, Y. / Kottur, J. / Fang, G. / Aggarwal, A.K.
External linksNat Commun / PubMed:40473611
MethodsX-ray diffraction
Resolution1.64 - 1.94 Å
Structure data

PDB-9dif:
CBASS Pseudomonas syringae Cap5 tetramer with DNA duplex and 3'2'-c-GAMP cyclic dinucleotide ligand
Method: X-RAY DIFFRACTION / Resolution: 1.67 Å

PDB-9dih:
CBASS Pseudomonas syringae Cap5 tetramer with DNA duplex and 3'2'-c-diAMP cyclic dinucleotide ligand
Method: X-RAY DIFFRACTION / Resolution: 1.94 Å

PDB-9nlg:
CBASS Pseudomonas syringae Cap5 tetramer with 3'2'-c-GAMP cyclic dinucleotide ligand (His56Ala mutant without Mg2+ ions)
Method: X-RAY DIFFRACTION / Resolution: 1.64 Å

Chemicals

ChemComp-4UR:
3'2'-cGAMP

ChemComp-ZN:
Unknown entry

ChemComp-HOH:
WATER


ChemComp, No image

ChemComp-Y4F:
Unknown entry

ChemComp-GOL:
GLYCEROL

Source
  • pseudomonas syringae (bacteria)
  • synthetic construct (others)
KeywordsIMMUNE SYSTEM / Bacterial immunity / CBASS / cyclic dinucleotide / Cap5 effector DNA endonuclease / viral defense / HNH endonuclease / DNA

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