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TitleCryo-EM structures of a Xanthomonas phage: Insights into viral architecture and implications for the model phage HK97.
Journal, issue, pagesStructure, Year 2025
Publish dateApr 15, 2025
AuthorsMingcheng Guo / Aohan Wang / Yaqi Zheng / Chaoying Liu / Qianqian Shao / Yunfei Deng / Lin Li / Yueting Wang / Xiaofang Wang / Yue Shen / Jun Qian / Xiaofeng Zhou / Qianglin Fang /
PubMed AbstractXanthomonas bacteria are responsible for disease outbreaks in several hundred plant species, causing significant economic losses. Xanthomonas phages have emerged as a promising biocontrol strategy in ...Xanthomonas bacteria are responsible for disease outbreaks in several hundred plant species, causing significant economic losses. Xanthomonas phages have emerged as a promising biocontrol strategy in managing various important plant diseases caused by Xanthomonas bacteria. However, structural information for Xanthomonas phages has remained limited so far. Here, we present high-resolution cryo-electron microscopy (cryo-EM) structures of the Xanthomonas citri phage ΦXacJX1 from siphoviruses. These structures include atomic models for the head, head-to-tail connector and head-proximal portion of the tail. ΦXacJX1's head and head-to-tail connector components show significant protein sequence and structural homology with those of the model siphophage HK97. However, the in-situ structures of head-to-tail connector of phage HK97 remain unavailable. The presented structures of phage ΦXacJX1 enhance our understanding of Xanthomonas phages and the mature virion of phage HK97. They provide a valuable framework for future structural and functional studies on both Xanthomonas phages and phage HK97.
External linksStructure / PubMed:40273907
MethodsEM (single particle)
Resolution3.45 - 3.78 Å
Structure data

EMDB-62947, PDB-9lbm:
Cryo-EM structure of bacteriophage phiXacJX1 capsid
Method: EM (single particle) / Resolution: 3.5 Å

EMDB-62948, PDB-9lbn:
The composite cryo-EM structure of the head-to-tail connector and head-proximal tail components of bacteriophage phiXacJX1
Method: EM (single particle) / Resolution: 3.6 Å

EMDB-62949: Cryo-EM structure of the head-to-tail connector and head-proximal tail components of bacteriophage phiXacJX1
Method: EM (single particle) / Resolution: 3.47 Å

EMDB-62951: Local refinement of bacteriophage phiXacJX1 head-to-tail connector
Method: EM (single particle) / Resolution: 3.45 Å

EMDB-62952: Local refinement of bacteriophage phiXacJX1 head-proximal tail tube
Method: EM (single particle) / Resolution: 3.78 Å

Source
  • xanthomonas phage phixacjx1 (virus)
KeywordsVIRUS / Xanthomonas phage / capsid / icosahedron / head-to-tail connector / tail

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