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TitleRNA-mediated CRISPR-Cas13 inhibition through crRNA structural mimicry.
Journal, issue, pagesScience, Vol. 388, Issue 6745, Page 387-391, Year 2025
Publish dateApr 25, 2025
AuthorsVictoria M Hayes / Jun-Tao Zhang / Mark A Katz / Yuelong Li / Benjamin Kocsis / David M Brinkley / Ning Jia / Alexander J Meeske /
PubMed AbstractTo circumvent CRISPR-Cas immunity, phages express anti-CRISPR factors that inhibit the expression or activities of Cas proteins. Whereas most anti-CRISPRs described to date are proteins, recently ...To circumvent CRISPR-Cas immunity, phages express anti-CRISPR factors that inhibit the expression or activities of Cas proteins. Whereas most anti-CRISPRs described to date are proteins, recently described small RNAs called RNA anti-CRISPRs (rAcrs) have sequence homology to CRISPR RNAs (crRNAs) and displace them from cognate Cas nucleases. In this work, we report the discovery of rAcrVIA1-a plasmid-encoded small RNA that inhibits the RNA-targeting CRISPR-Cas13 system in its natural host, . We solved the cryo-electron microscopy structure of the Cas13-rAcr complex, which revealed that rAcrVIA1 adopts a fold nearly identical to crRNA despite sharing negligible sequence similarity. Collectively, our findings expand the diversity of rAcrs and reveal an example of immune antagonism through RNA structural mimicry.
External linksScience / PubMed:40273258 / PubMed Central
MethodsEM (single particle)
Resolution2.85 Å
Structure data

60476

8zty

EMDB-60476, PDB-8zty:
Cryo-EM structure of the Cas13a-rAcrVIA1 complex
Method: EM (single particle) / Resolution: 2.85 Å

Source
  • listeria seeligeri serovar 1/2b str. slcc3954 (bacteria)
KeywordsRNA BINDING PROTEIN/RNA / STRUCTURAL PROTEIN / RNA BINDING PROTEIN-RNA complex

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