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-Structure paper
| タイトル | Structure of the turnip yellows virus particles. |
|---|---|
| ジャーナル・号・ページ | Virology, Vol. 607, Page 110514, Year 2025 |
| 掲載日 | 2025年3月26日 |
著者 | Joséphine Lai-Kee-Him / Stefano Trapani / Sylvaine Boissinot / Catherine Reinbold / Chloé Fallet / Aurélie Ancelin / François Lecorre / François Hoh / Véronique Ziegler-Graff / Véronique Brault / Patrick Bron / ![]() |
| PubMed 要旨 | Turnip yellows virus (TuYV) is a plant virus infecting important crops such as oilseed rape. TuYV is phloem-restricted and transmitted by aphids. The capsid contains two subunit types: the major ...Turnip yellows virus (TuYV) is a plant virus infecting important crops such as oilseed rape. TuYV is phloem-restricted and transmitted by aphids. The capsid contains two subunit types: the major capsid protein (CP) and a minor component (RTP∗) which arises from the C-terminal cleavage of a readthrough product (RTP). RTP∗ contains the CP sequence fused with a structured domain, denoted RTD, which is a key determinant of virus transmission. Though both CP and RTP∗ are involved in virus movement and aphid transmission, how RTP∗ is incorporated into the capsid is poorly understood. We present here the structural characterisation, by immunogold labelling and 3D cryo-EM, of the wild-type TuYV and a mutant whose capsid contains the CP only. We show that incorporation of RTP∗ does not impair the capsid structure, and the RTD does not adopt well-defined positions at the capsid surface. The number of incorporated RTP∗s suggests a random insertion. |
リンク | Virology / PubMed:40179450 |
| 手法 | EM (単粒子) |
| 解像度 | 3.47 - 4.08 Å |
| 構造データ | EMDB-10003, PDB-9fhp: ![]() PDB-9q8j: |
| 由来 |
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キーワード | VIRUS / read-through protein |
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